Characterization of dielectrophoresis-aligned nanofibrous silk fibroin-chitosan scaffold and its interactions with endothelial cells for tissue engineering applications.
Characterization of dielectrophoresis-aligned nanofibrous silk fibroin-chitosan scaffold and its interactions with endothelial cells for tissue engineering applications.
复制标题
DOI：
10.1016/j.actbio.2014.05.005
复制
发表时间：
2014-08
影响因子：
9.7
通讯作者：
Mathur, Anshu B.
中科院分区：
文献类型：
--
作者：
Dunne, Lina W.;Iyyanki, Tejaswi;Hubenak, Justin;Mathur, Anshu B.
研究了交流电频率、离子存在、SF/CS比和DEP冷冻温度对三维定向纳米丝素-壳聚糖(ESFCS)支架性能的影响。用偏光显微镜(PLM)分析SF高分子链的排列，原子力显微镜(AFM)测量表观弹性模数，扫描电子显微镜和AFM分析支架的形貌。采用免疫组织化学染色检测细胞构型，原子力显微镜(AFM)测量细胞表观弹性模量值，评价人脐静脉内皮细胞(HUVECs)与eSFCS支架的相互作用。与其他条件相比，在10 MHz下用氯化钠制备的eSFCS(50：50)样品具有最高的排列面积百分比。随着DEP频率从100 kHz增加到10 MHz，原纤维尺寸显着减小。在5 mM氯化钠存在下，以10 MHz频率制备的eSFCS(50：50)支架的纤维尺寸为77.96±4.69 nm，表观弹性模量为39.9±22.4kPa.人脐静脉内皮细胞在eSFCS支架上形成排列整齐的分支毛细血管样结构。人脐静脉内皮细胞在eSFCS上的弹性模数为6.36±2.37kPa.DEP是一种潜在的构建具有定向纳米纤维结构的SFCS支架的工具，可以指导组织工程和修复中的血管形成。
Aligned three-dimensional nanofibrous silk fibroin-chitosan (eSFCS) scaffolds were fabricated using dielectrophoresis (DEP) by investigating the effects of alternating current frequency, the presence of ions, SF:CS ratio, and post-DEP freezing temperature. Scaffolds were characterized with polarized light microscopy (PLM) to analyze SF polymer chain alignment, atomic force microscopy (AFM) to measure the apparent elastic modulus, and scanning electron microscopy and AFM to analyze scaffold topography. The interaction of human umbilical vein endothelial cells (HUVECs) with eSFCS scaffolds was assessed using immunostaining to assess cell patterning and AFM to measure the apparent elastic modulus of the cells. The eSFCS (50:50) samples prepared at 10 MHz with NaCl had the highest percentage of aligned area as compared to other conditions. As DEP frequency increased from 100 kHz to 10 MHz fibril sizes decreased significantly. eSFCS (50:50) scaffolds fabricated at 10 MHz in the presence of 5 mM NaCl had a fibril size of 77.96 ± 4.69 nm and an apparent elastic modulus of 39.9 ± 22.4 kPa. HUVECs on eSFCS scaffolds formed aligned and branched capillary-like vascular structures. The elastic modulus of HUVEC cultured on eSFCS was 6.36 ± 2.37 kPa. DEP is a potential tool for fabrication of SFCS scaffolds with aligned nanofibrous structures that can guide vasculature in tissue engineering and repair.
登录
查看更多内容
时间分辨傅立叶变换红外光谱监测再生家蚕丝素蛋白膜的构象转变动力学
影响因子：
3.8
作者：
Chen, X;Shao, ZZ;Chance, MR
通讯作者：
Chance, MR
影响因子：
2.4
作者：
Mathur, AB;Collinsworth, AM;Truskey, GA
通讯作者：
Truskey, GA
影响因子：
3.9
作者：
Bakewell, DJ;Morgan, H
通讯作者：
Morgan, H
影响因子：
10.8
作者：
Asokan, SB;Jawerth, L;Superfine, R
通讯作者：
Superfine, R
影响因子：
14
作者：
Lovett, Michael;Cannizzaro, Christopher;Kaplan, David L.
通讯作者：
Kaplan, David L.