Pigment Dispersing Factors and Their Cognate Receptors in a Crustacean Model, With New Insights Into Distinct Neurons and Their Functions.
Pigment Dispersing Factors and Their Cognate Receptors in a Crustacean Model, With New Insights Into Distinct Neurons and Their Functions.
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DOI：
10.3389/fnins.2020.595648
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发表时间：
2020
影响因子：
4.3
通讯作者：
Webster SG
中科院分区：
文献类型：
--
作者：
Alexander JL;Oliphant A;Wilcockson DC;Brendler-Spaeth T;Dircksen H;Webster SG
色素分散因子（PDFs，或甲壳类动物中的PDHs）形成了一组结构相关的神经肽，在整个蜕皮动物中发现，并首次在甲壳类动物中作为色素效应激素被发现。在昆虫中，PDF履行关键的神经调节作用，最显着的是作为昼夜节律系统的输出调节器，强调其在节肢动物的生理和行为组织中的中心地位。有趣的是，十足类甲壳动物表达源自不同基因的多种HH亚型，但它们的差异功能仍有待确定。在这里，我们在功能上定义了两个PDH受体的蟹巨蟹座，并显示它们被选择性激活的四个PDH亚型：PDHR 43673被激活的PDH-1和PDH-2在低纳摩尔剂量，而PDHR 41189被激活的PDH-3和一个扩展的20个残基的e-PDH。对所有四种肽及其同源受体的解剖分布的详细检查表明，它们可能作为分泌的激素和/或神经调节剂执行不同的功能，其中PDH-1及其受体43，673涉及真实的激素轴。PDH-2、PDH-3和e-PDH仅限于CNS中的非神经血管神经元间位点; PDHR 41189主要限于神经系统，表明具有神经调节功能。值得注意的是，PDH-3和e-PDH没有色素细胞分散活性。这是第一份报告，在功能上定义了PDHR内分泌系统中的甲壳类动物，并指出这一点和其他假定的作用，这一生理关键肽组在这些生物体。因此，我们的研究结果提供了机会，以进一步研究内分泌和昼夜机制，在这个重要的节肢动物门。
Pigment dispersing factors (PDFs, or PDHs in crustaceans) form a structurally related group of neuropeptides found throughout the Ecdysozoa and were first discovered as pigmentary effector hormones in crustaceans. In insects PDFs fulfill crucial neuromodulatory roles, most notably as output regulators of the circadian system, underscoring their central position in physiological and behavioral organization of arthropods. Intriguingly, decapod crustaceans express multiple isoforms of PDH originating from separate genes, yet their differential functions are still to be determined. Here, we functionally define two PDH receptors in the crab Carcinus maenas and show them to be selectively activated by four PDH isoforms: PDHR 43673 was activated by PDH-1 and PDH-2 at low nanomolar doses whilst PDHR 41189 was activated by PDH-3 and an extended 20 residue e-PDH. Detailed examination of the anatomical distribution of all four peptides and their cognate receptors indicate that they likely perform different functions as secreted hormones and/or neuromodulators, with PDH-1 and its receptor 43,673 implicated in an authentic hormonal axis. PDH-2, PDH-3, and e-PDH were limited to non-neurohemal interneuronal sites in the CNS; PDHR 41189 was largely restricted to the nervous system suggesting a neuromodulatory function. Notably PDH-3 and e-PDH were without chromatophore dispersing activity. This is the first report which functionally defines a PDHR in an endocrine system in a crustacean and to indicate this and other putative roles of this physiologically pivotal peptide group in these organisms. Thus, our findings present opportunities to further examine the endocrine and circadian machinery in this important arthropod phylum.
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影响因子：
4.3
作者：
Alexander J;Oliphant A;Wilcockson DC;Webster SG
通讯作者：
Webster SG
影响因子：
5.2
作者：
Caers J;Verlinden H;Zels S;Vandersmissen HP;Vuerinckx K;Schoofs L
通讯作者：
Schoofs L
DOI：
10.1016/0005-2795(76)90155-0
发表时间：
1976-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FERNLUND, P
通讯作者：
FERNLUND, P
影响因子：
4.6
作者：
Goda, Tadahiro;Umezaki, Yujiro;Hamada, Fumika N.
通讯作者：
Hamada, Fumika N.
影响因子：
3.6
作者：
Elekes, K;Nässel, DR
通讯作者：
Nässel, DR