Functional Identification and Characterization of the Diuretic Hormone 31 (DH31) Signaling System in the Green Shore Crab, Carcinus maenas.
Functional Identification and Characterization of the Diuretic Hormone 31 (DH31) Signaling System in the Green Shore Crab, Carcinus maenas.
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DOI：
10.3389/fnins.2018.00454
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发表时间：
2018
影响因子：
4.3
通讯作者：
Webster SG
中科院分区：
文献类型：
--
作者：
Alexander J;Oliphant A;Wilcockson DC;Webster SG
甲壳动物神经肽及其同源受体的功能表征没有跟上最近的进展，在序列测定，因此，我们的理解在这个重要的节肢动物亚门的神经肽的生理作用是相当有限的。我们确定了一个候选受体配体配对利尿激素31（DH 31）的神经转录组的螃蟹，巨蟹。在昆虫中，DH 31在生理学的许多方面（包括流体分泌、肌肉活动和肠道蠕动）中发挥物种特异性但核心的作用，但关于其在甲壳类动物中的功能知之甚少。梭maenas DH 31转录物编码147个氨基酸的前原肽，并且单个受体转录物翻译为促分泌素样（B1类）G蛋白偶联受体（GPCR）。我们使用体外水母发光Ca 2+动员试验来证明该候选DH 31 R以剂量依赖性方式（EC 50 15-30 nM）被癌肿和昆虫DH 31 s激活。全坐骑免疫组织化学和原位杂交定位显示广泛的DH 31表达神经元在整个中枢神经系统，最显着的是在腹神经节，其中大的，未配对的细胞产生内侧神经，终止于广泛的DH 31免疫阳性树突状领域密切相关的食管肌肉组织。该系统构成了一个大的和迄今为止未描述的神经血管区，邻近与胃系统相关的关键肌群。DH 31表达神经元也被认为是在心脏，连合，食管，和口胃神经节和强烈的标签被认为是在树突支配前和后肠肌肉组织，但没有肢体肌肉。这些标记模式，连同心脏和后肠中DH 31 R mRNA的测量，促使我们测试DH 31对半分离心脏制备物的影响。用肽进行心脏灌流引起心率增加（10-100 nM）。DH 31及其受体转录物的神经解剖学分布，特别是与胃和心脏肌肉组织相关的分布，加上肽的心脏加速作用，暗示该肽具有关键的肌活性作用，可能与节律协调有关。
The functional characterization of crustacean neuropeptides and their cognate receptors has not kept pace with the recent advances in sequence determination and, therefore, our understanding of the physiological roles of neuropeptides in this important arthropod sub-phylum is rather limited. We identified a candidate receptor-ligand pairing for diuretic hormone 31 (DH31) in a neural transcriptome of the crab, Carcinus maenas. In insects, DH31 plays species -specific but central roles in many facets of physiology, including fluid secretion, myoactivity, and gut peristalsis but little is known concerning its functions in crustaceans. The C. maenas DH31 transcript codes for a 147 amino acid prepropeptide, and a single receptor transcript translates to a secretin-like (Class B1) G protein-coupled receptor (GPCR). We used an in vitro aequorin luminescence Ca2+ mobilization assay to demonstrate that this candidate DH31R is activated byCarcinus and insect DH31s in a dose-dependent manner (EC50 15–30 nM). Whole mount immunohistochemical and in situ hybridization localization revealed extensive DH31 expressing neurons throughout the central nervous system, most notably in the abdominal ganglion where large, unpaired cells give rise to medial nerves, which terminate in extensive DH31 immunopositive dendritic fields intimately associated with oesophageal musculature. This system constitutes a large and hitherto undescribed neurohemal area adjacent to key muscle groups associated with the gastric system. DH31 expressing neurons were also seen in the cardiac, commissural, oesophageal, and stomatogastric ganglia and intense labeling was seen in dendrites innervating fore- and hindgut musculature but not with limb muscles. These labeling patterns, together with measurement of DH31R mRNA in the heart and hindgut, prompted us test the effects of DH31 on semi-isolated heart preparations. Cardiac superfusion with peptide evoked increased heart rates (10–100 nM). The neuroanatomical distribution of DH31 and its receptor transcripts, particularly that associated with gastric and cardiac musculature, coupled with the cardio- acceleratory effects of the peptide implicate this peptide in key myoactive roles, likely related to rhythmic coordination.
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影响因子：
5.2
作者：
Caers J;Verlinden H;Zels S;Vandersmissen HP;Vuerinckx K;Schoofs L
通讯作者：
Schoofs L
影响因子：
3
作者：
Brugge, V. A. Te;Orchard, I.
通讯作者：
Orchard, I.
影响因子：
2.7
作者：
Hoelters, Laura;O'Grady, Joseph Francis;Wilcockson, David Charles
通讯作者：
Wilcockson, David Charles
DOI：
10.1126/science.1197761
发表时间：
2011-02-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colbourne JK;Pfrender ME;Gilbert D;Thomas WK;Tucker A;Oakley TH;Tokishita S;Aerts A;Arnold GJ;Basu MK;Bauer DJ;Cáceres CE;Carmel L;Casola C;Choi JH;Detter JC;Dong Q;Dusheyko S;Eads BD;Fröhlich T;Geiler-Samerotte KA;Gerlach D;Hatcher P;Jogdeo S;Krijgsveld J;Kriventseva EV;Kültz D;Laforsch C;Lindquist E;Lopez J;Manak JR;Muller J;Pangilinan J;Patwardhan RP;Pitluck S;Pritham EJ;Rechtsteiner A;Rho M;Rogozin IB;Sakarya O;Salamov A;Schaack S;Shapiro H;Shiga Y;Skalitzky C;Smith Z;Souvorov A;Sung W;Tang Z;Tsuchiya D;Tu H;Vos H;Wang M;Wolf YI;Yamagata H;Yamada T;Ye Y;Shaw JR;Andrews J;Crease TJ;Tang H;Lucas SM;Robertson HM;Bork P;Koonin EV;Zdobnov EM;Grigoriev IV;Lynch M;Boore JL
通讯作者：
Boore JL
影响因子：
4.4
作者：
Conzelmann M;Williams EA;Krug K;Franz-Wachtel M;Macek B;Jékely G
通讯作者：
Jékely G