Msh Pilus Mutations Increase the Ability of a Free-Living Bacterium to Colonize a Piscine Host.
Msh Pilus Mutations Increase the Ability of a Free-Living Bacterium to Colonize a Piscine Host.
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DOI：
10.3390/genes12020127
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发表时间：
2021-01-20
期刊：
影响因子：
3.5
通讯作者：
Bohannan BJM
中科院分区：
文献类型：
--
作者：
Lebov JF;Bohannan BJM
动物和细菌之间的共生体无处不在。为了更好地理解这些关系，有必要解开细菌如何进化以殖民宿主。在此之前，我们连续传代的自由生活的细菌，希瓦氏菌oneidensis，通过消化道的无菌幼虫斑马鱼（Danio rerio），揭示了进化的变化，参与启动一种新的共生与脊椎动物的主机。20代后，我们发现了一个自适应的mshL基因的msh菌毛操纵子，提高主机殖民，增加游泳运动，减少表面粘附的错义突变。在目前的研究中，我们确定该突变是一种功能缺失突变，并发现它通过增加S. oneidensis表示在水柱外幼虫通过减少与环境表面的联系。此外，我们发现含有mshL突变的菌株能够以较高的人均速率迁移到宿主消化道中。然而，突变体和进化菌株没有表现出任何证据的竞争优势后，殖民主机。我们的研究结果表明，宿主外的细菌行为可以发挥主导作用，促进新的主机协会的发病。
Symbioses between animals and bacteria are ubiquitous. To better understand these relationships, it is essential to unravel how bacteria evolve to colonize hosts. Previously, we serially passaged the free-living bacterium, Shewanella oneidensis, through the digestive tracts of germ-free larval zebrafish (Danio rerio) to uncover the evolutionary changes involved in the initiation of a novel symbiosis with a vertebrate host. After 20 passages, we discovered an adaptive missense mutation in the mshL gene of the msh pilus operon, which improved host colonization, increased swimming motility, and reduced surface adhesion. In the present study, we determined that this mutation was a loss-of-function mutation and found that it improved zebrafish colonization by augmenting S. oneidensis representation in the water column outside larvae through a reduced association with environmental surfaces. Additionally, we found that strains containing the mshL mutation were able to immigrate into host digestive tracts at higher rates per capita. However, mutant and evolved strains exhibited no evidence of a competitive advantage after colonizing hosts. Our results demonstrate that bacterial behaviors outside the host can play a dominant role in facilitating the onset of novel host associations.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
6.7
作者：
Jones CJ;Utada A;Davis KR;Thongsomboon W;Zamorano Sanchez D;Banakar V;Cegelski L;Wong GC;Yildiz FH
通讯作者：
Yildiz FH
影响因子：
4.5
作者：
Hottes AK;Freddolino PL;Khare A;Donnell ZN;Liu JC;Tavazoie S
通讯作者：
Tavazoie S
影响因子：
6.4
作者：
Chen, Yi-Hsuan;Li, Shao-Hui;Chang, Yung-Chi
通讯作者：
Chang, Yung-Chi
影响因子：
2.7
作者：
Bates, Jennifer M.;Mittge, Erika;Guillemin, Karen
通讯作者：
Guillemin, Karen