Relationship between the CAG Repeat Polymorphism in the Androgen Receptor Gene and Acne in the Han Ethnic Group
Relationship between the CAG Repeat Polymorphism in the Androgen Receptor Gene and Acne in the Han Ethnic Group
复制标题
汉族雄激素受体基因CAG重复多态性与痤疮的关系
作者：
Yang, Zhi;Yu, Haijing;Cheng, Baowen;Dong, Yongli;Xiao, Chunjie;Tang, Wenru;He, Li
背景资料：人雄激素受体（AR）基因的调节域包含一个多态性CAG重复序列，编码与AR转录活性呈负相关的多聚谷氨酰胺束。雄激素通过AR发挥作用，在寻常痤疮的发病机制中起着至关重要的作用。因此，我们研究了AR基因CAG重复多态性与痤疮易感性之间的关系。方法：对206例痤疮患者和200例正常对照者进行研究。从患者外周血淋巴细胞中提取基因组DNA，使用荧光标记引物通过聚合酶链反应（PCR）扩增CAG重复序列。然后将样品在具有内部分子量标记的ABI 377基因扫描分析凝胶上运行。随机选择10个男性样本进行测序，以确认CAG重复序列的数量。采用双样本独立t检验进行数据分析。结果：正常对照组AR区CAG重复序列平均数为22.07（14-28）个，男性痤疮组为20.61（13-26）个。CAG重复序列长度与男性痤疮发病有显著相关性。女性患者与对照组之间无显著差异。结论：结果提示AR基因CAG重复序列多态性可能是汉族男性痤疮易感性的候选遗传标记之一。
Background: The modulatory domain of the human androgen receptor (AR) gene contains a polymorphic CAG repeat coding for a polyglutamine tract which is inversely correlated with transcriptional activity of the AR. Androgens acting through the AR play a crucial role in the pathogenesis of acne vulgaris. We therefore investigated the relationship between CAG repeat polymorphism in the AR gene and acne susceptibility. Methods: 206 acne patients and 200 controls participated in the study. Genomic DNA was extracted from peripheral blood lymphocytes of individual patients, and the CAG repeat region was amplified by polymerase chain reaction (PCR) using fluorescence-labeled primers. Samples were then run on an ABI 377 gene scan analysis gel with an internal molecular-weight marker. Ten male samples were chosen randomly for sequencing to confirm the number of CAG repeats. The 2-sample independent t test was used to analyze the data. Results: The mean number of the CAG repeat in the AR was 22.07 (14–28) in the controls and 20.61 (13–26) in the male acne group. There was a significant correlation between the CAG repeat length and male acne. No significant difference was observed between female patients and their controls. Conclusion: The results suggest that the AR gene CAG repeat polymorphism may be one of the candidate genetic markers for male acne susceptibility in the Han population.
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影响因子：
7.2
作者：
Adesanya-Famuyiwa, OO;Zhou, JA;Bondy, C
通讯作者：
Bondy, C
DOI：
10.1111/1523-1747.ep12472330
发表时间：
1993-05
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Tehming Liang;Sheryl Hoyer;Riley Yu;Keyoumars Soltani;Allan L. Lorincz;R. Hiipakka;S. Liao
通讯作者：
Tehming Liang;Sheryl Hoyer;Riley Yu;Keyoumars Soltani;Allan L. Lorincz;R. Hiipakka;S. Liao
DOI：
10.1111/j.1432-1033.1973.tb02881.x
发表时间：
1973-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GROSS-BELLARD, MM;OUDET, P;CHAMBON, P
通讯作者：
CHAMBON, P
影响因子：
11.2
作者：
J. Stanford;J. Just;M. Gibbs;K. Wicklund;C. Neal;B. Blumenstein;E. Ostrander
通讯作者：
J. Stanford;J. Just;M. Gibbs;K. Wicklund;C. Neal;B. Blumenstein;E. Ostrander
影响因子：
4.1
作者：
Panet-Raymond, V;Gottlieb, B;Trifiro, MA
通讯作者：
Trifiro, MA