Tumour necrosis factor-α exerts dual effects on human adipose leptin synthesis and release
Tumour necrosis factor-α exerts dual effects on human adipose leptin synthesis and release
复制标题
肿瘤坏死因子-α 对人体脂肪瘦素合成和释放具有双重作用
DOI：
10.1016/s0303-7207(99)00194-x
复制
发表时间：
2000
影响因子：
4.1
通讯作者：
M. Eggo
中科院分区：
文献类型：
--
作者：
Hui H. Zhang;Sudhesh Kumar;A. Barnett;M. Eggo
采用竞争性RT-PCR和Western blotting方法研究了肿瘤坏死因子-α（TNF-α）对人前脂肪细胞、分化前脂肪细胞和成熟脂肪细胞产生瘦素的急性和慢性效应。在前脂肪细胞中，分泌的瘦素在分化培养基中孵育5天后可检测到，并且在第20天增加了4倍。TNF-α在分化过程中阻断瘦素的合成。在分化的前脂肪细胞和成熟脂肪细胞中，TNF-α处理导致瘦素和甘油-3-磷酸脱氢酶（G3 PD）mRNA的时间依赖性降低。相反，TNF-α（4-8小时处理）导致瘦素释放增加4倍。这种作用在24 h时消失，加入TNF-α后24-48 h，培养基中瘦素的积累减少。我们的结论是，TNF-α刺激人成熟脂肪细胞和现有分化的前脂肪细胞释放预先形成的瘦素，这可能有助于肥胖/感染相关的高瘦素血症，TNF-α通过抑制前脂肪细胞分化和诱导脂肪细胞去分化来抑制瘦素合成。
The acute and chronic effects of tumour necrosis factor-α (TNF-α) on leptin production by human preadipocytes, differentiated preadipocytes, and mature adipocytes have been examined by competitive RT-PCR of leptin mRNA and by western blotting. In preadipocytes, secreted leptin was detectable after 5-day incubation in differentiation medium and this increased 4-fold by day 20. TNF-α blocked leptin synthesis during differentiation. In differentiated preadipocytes and mature adipocytes, TNF-α treatment resulted in time-dependent decreases in mRNA for leptin and glycerol-3-phosphate dehydrogenase (G3PD). In contrast, TNF-α (4–8-h treatment) resulted in a 4-fold increase in leptin release. This effect was lost at 24 h and leptin accumulation in culture medium was decreased 24–48 h after TNF-α addition. We conclude that TNF-α stimulates the release of preformed leptin from human mature adipocytes and existing differentiated preadipocytes, which may contribute to obesity/infection-linked hyperleptinemia, and that TNF-α inhibits leptin synthesis via inhibition of preadipocyte differentiation and induction of adipocyte dedifferentiation.
登录
查看更多内容
影响因子：
--
作者：
M. Pape;K. H. Kim
通讯作者：
M. Pape;K. H. Kim
肿瘤坏死因子-α的脂肪表达--在与肥胖相关的胰岛素抵抗中的直接作用
影响因子：
56.9
作者：
HOTAMISLIGIL, GS;SHARGILL, NS;SPIEGELMAN, BM
通讯作者：
SPIEGELMAN, BM
DOI：
10.1210/jcem.81.11.8923837
发表时间：
1996-11
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
R. Ostlund;Joseph W. Yang;S. Klein;R. Gingerich
通讯作者：
R. Ostlund;Joseph W. Yang;S. Klein;R. Gingerich
影响因子：
3.5
作者：
STEPHENS, JM;BUTTS, MD;PEKALA, PH
通讯作者：
PEKALA, PH
DOI：
10.1016/s0021-9258(18)54714-1
发表时间：
1991-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jacqueline M. Stephens;P. Pekala
通讯作者：
Jacqueline M. Stephens;P. Pekala