Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration.
Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration.
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斑马鱼Sox9b对于肝癌的发育和胰腺内分泌细胞再生至关重要。
DOI：
10.1016/j.ydbio.2012.04.002
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发表时间：
2012-06-15
影响因子：
2.7
通讯作者：
Peers B
中科院分区：
文献类型：
--
作者：
Manfroid I;Ghaye A;Naye F;Detry N;Palm S;Pan L;Ma TP;Huang W;Rovira M;Martial JA;Parsons MJ;Moens CB;Voz ML;Peers B
Recent zebrafish studies have shown that the late appearing pancreatic endocrine cells derive from pancreatic ducts but the regulatory factors involved are still largely unknown. Here, we show that the zebrafish sox9b gene is expressed in pancreatic ducts where it labels the pancreatic Notch-responsive cells previously shown to be progenitors. Inactivation of sox9b disturbs duct formation and impairs regeneration of beta cells from these ducts in larvae. sox9b expression in the midtrunk endoderm appears at the junction of the hepatic and ventral pancreatic buds and, by the end of embryogenesis, labels the hepatopancreatic ductal system as well as the intrapancreatic and intrahepatic ducts. Ductal morphogenesis and differentiation are specifically disrupted in sox9b mutants, with the dysmorphic hepatopancreatic ducts containing misdifferentiated hepatocyte-like and pancreatic-like cells. We also show that maintenance of sox9b expression in the extrapancreatic and intrapancreatic ducts requires FGF and Notch activity, respectively, both pathways known to prevent excessive endocrine differentiation in these ducts. Furthermore, beta cell recovery after specific ablation is severely compromised in sox9b mutant larvae. Our data position sox9b as a key player in the generation of secondary endocrine cells deriving from pancreatic ducts in zebrafish.
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影响因子：
2.6
作者：
Korzh, S;Emelyanov, A;Korzh, V
通讯作者：
Korzh, V
DOI：
10.1073/pnas.0704054104
发表时间：
2007-06-19
影响因子：
11.1
作者：
Lynn, F. C.;Smith, S. B.;German, M. S.
通讯作者：
German, M. S.
影响因子：
2.7
作者：
Chiang, EFL;Pai, CI;Chung, BC
通讯作者：
Chung, BC
DOI：
10.1073/pnas.0504750102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Akiyama, H;Kim, JE;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
DOI：
10.1002/dvdy.22220
发表时间：
2010-03
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
Lorent K;Moore JC;Siekmann AF;Lawson N;Pack M
通讯作者：
Pack M