Tissue engineering of acellular vascular grafts capable of somatic growth in young lambs.
Tissue engineering of acellular vascular grafts capable of somatic growth in young lambs.
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DOI：
10.1038/ncomms12951
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发表时间：
2016-09-27
影响因子：
16.6
通讯作者：
Tranquillo, Robert T.
中科院分区：
文献类型：
--
作者：
Syedain, Zeeshan;Reimer, Jay;Lahti, Matthew;Berry, James;Johnson, Sandra;Tranquillo, Robert T.
需要右心室流出道重建的儿童先天性心脏病的治疗通常涉及多次心内直视手术，因为所有现有的移植材料都没有生长潜力。在这里，我们提出了一个'现成的'血管移植生长从供体成纤维细胞在纤维蛋白凝胶，以解决这一关键的未满足的需求。在一项概念验证研究中，将脱细胞移植物作为肺动脉置换物植入三只年轻羔羊，并对其成年后进行评估。纵向超声记录移植物的尺寸生长。羔羊生长正常，体重增加366%，移植物直径和体积分别增加56%和216%。取出的移植物显示出生理强度和刚度，完全管腔内皮化和成熟平滑肌细胞的广泛群体。移植物还显示大量弹性蛋白沉积和胶原蛋白含量增加465%，没有钙化、动脉瘤或狭窄的迹象。总的来说，我们的数据支持这种完全生物移植的体细胞生长。 目前的血管移植物必须手术更换时，受体超越他们。在这里，Syedain等生物工程师从绵羊成纤维细胞中产生了一种无细胞基质管，当移植到生长中的羔羊时，这种基质能够细胞化和体细胞生长，从而消除了多次移植手术的需要。
Treatment of congenital heart defects in children requiring right ventricular outflow tract reconstruction typically involves multiple open-heart surgeries because all existing graft materials have no growth potential. Here we present an ‘off-the-shelf' vascular graft grown from donor fibroblasts in a fibrin gel to address this critical unmet need. In a proof-of-concept study, the decellularized grafts are implanted as a pulmonary artery replacement in three young lambs and evaluated to adulthood. Longitudinal ultrasounds document dimensional growth of the grafts. The lambs show normal growth, increasing body weight by 366% and graft diameter and volume by 56% and 216%, respectively. Explanted grafts display physiological strength and stiffness, complete lumen endothelialization and extensive population by mature smooth muscle cells. The grafts also show substantial elastin deposition and a 465% increase in collagen content, without signs of calcification, aneurysm or stenosis. Collectively, our data support somatic growth of this completely biological graft. Current vessel grafts must be surgically replaced when the recipient outgrows them. Here, Syedain et al. bioengineer a tube of acellular matrix produced from sheep fibroblasts that is capable of cellularizaton and somatic growth when transplanted into growing lambs, eliminating the need for multiple graft surgeries.
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DOI：
10.1053/j.semtcvs.2014.08.002
发表时间：
2014-06-01
影响因子：
2.5
作者：
DiBardino, Daniel J.;Jacobs, Jeffrey P.
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DOI：
10.1016/j.jtcvs.2009.09.057
发表时间：
2010-02-01
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