Identification and quantitation of unsaturated fatty acid isomers by electrospray ionization tandem mass spectrometry: a shotgun lipidomics approach.
Identification and quantitation of unsaturated fatty acid isomers by electrospray ionization tandem mass spectrometry: a shotgun lipidomics approach.
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DOI：
10.1021/ac2006119
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发表时间：
2011-06-01
影响因子：
7.4
通讯作者：
Han, Xianlin
中科院分区：
文献类型：
--
作者：
Yang, Kui;Zhao, Zhongdan;Gross, Richard W.;Han, Xianlin
在生物系统中对不饱和脂肪酸（FA）异构体进行鉴定和定量，在脂质代谢和分解代谢、膜生物物理学以及疾病发病机制的研究中具有重要意义，但在脂质组学中具有挑战性。我们利用两个事实开发了一种鉴定和定量不饱和FA异构体的新方法：（1）不饱和FA阴离子在碰撞诱导解离时会因阴离子失去CO₂或H₂O而产生碎片离子；（2）不同的FA异构体产生的碎片离子在碎片离子强度与碰撞条件的关系上具有不同的特征图谱。这些不同的特征图谱可能是由于碎片离子的负电荷与双键的电子云因在不同的FA异构体中距离不同而产生的相互作用差异所致。这种新方法还通过多级串联质谱法扩展到分析磷脂中存在的FA链的双键异构体。总之，我们开发了一种鉴定和定量内源性FA种类或完整磷脂种类中存在的FA链的双键异构体的新方法。我们相信这种方法将进一步提高脂质组学在鉴定代谢疾病潜在生化机制方面的能力。
Identification and quantification of unsaturated fatty acid (FA) isomers in a biological system are significant in the study of lipid metabolism and catabolism, membrane biophysics, and pathogenesis of diseases, but are challenging in lipidomics. We developed a novel approach for identification and quantitation of unsaturated FA isomers by exploiting two facts: (1) unsaturated FA anions yield fragment ion(s) from loss of CO2 or H2O from the anions upon collision-induced dissociation; and (2) the fragment ions yielded from discrete FA isomers have distinct profiles of the fragment ion intensity vs. collision conditions. These distinct profiles likely result from the differential interactions of the negative charge of the fragment ion with the electron clouds of the double bonds due to their different distances in discrete FA isomers. The novel approach was also extended to analyze the double bond isomers of FA chains present in phospholipids by multi-stage tandem mass spectrometry. Collectively, we developed a new approach for identification and quantification of the double bond isomers of endogenous FA species or FA chains present in intact phospholipid species. We believe that this approach should further advance the lipidomic power for identification of the biochemical mechanisms underlying metabolic diseases.
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影响因子：
6.5
作者：
Pruett ST;Bushnev A;Hagedorn K;Adiga M;Haynes CA;Sullards MC;Liotta DC;Merrill AH Jr
通讯作者：
Merrill AH Jr
影响因子：
2.1
作者：
Han, Xianlin
通讯作者：
Han, Xianlin
DOI：
10.1016/s1044-0305(99)00035-5
发表时间：
1999-07-01
影响因子：
3.2
作者：
Hsu, FF;Turk, J
通讯作者：
Turk, J
影响因子：
7.4
作者：
BRYANT, DK;ORLANDO, RC;HENDERSON, LE
通讯作者：
HENDERSON, LE
通过多阶段线性离子陷阱质谱法用电喷雾电离阐明长链不饱和脂肪酸的双键位置。
DOI：
10.1016/j.jasms.2008.07.007
发表时间：
2008-11
影响因子：
3.2
作者：
Hsu FF;Turk J
通讯作者：
Turk J