Comparison of the roles of house dust mite allergens, ovalbumin and lipopolysaccharides in the sensitization of mice to establish a model of severe neutrophilic asthma.
Comparison of the roles of house dust mite allergens, ovalbumin and lipopolysaccharides in the sensitization of mice to establish a model of severe neutrophilic asthma.
复制标题
屋尘螨过敏原、卵清蛋白和脂多糖对小鼠致敏建立重度中性粒细胞性哮喘模型的作用比较
DOI：
10.3892/etm.2017.4776
复制
发表时间：
2017-09
影响因子：
2.7
通讯作者：
Xiang X
中科院分区：
文献类型：
--
作者：
Jia A;Wang Y;Sun W;Xiao B;Mu L;Wei Y;Xu L;Peng C;Zhang D;Shen H;Xiang X
嗜中性粒细胞性哮喘（NA）与严重的病程和对皮质类固醇的不良反应有关。本研究旨在比较不同浓度的屋尘螨（HDM）过敏原，卵清蛋白（OVA），主要鸡蛋过敏原，和脂多糖（LPS）的组合对严重NA的发病的影响。根据随机数字表对雌性C57 BL/6小鼠进行分组，并在研究的第0、1和2天用HDM/LPS/OVA提取物鼻内致敏。在第1组中，小鼠接受50 μg HDM + 50 μg OVA + 15 μg LPS，第2组中的小鼠接受50 μg HDM + 100 μg OVA + 15 μg LPS，第3组中的小鼠接受100 μg HDM + 50 μg OVA + 15 μg LPS，第4组中的小鼠接受100 μg HDM + 100 μg OVA + 15 μg LPS，而对照组的小鼠仅接受盐水。然后在第14、15、18、19和20天用雾化激发的OVA溶液攻击小鼠，每次30 min。观察动物行为学、气道高反应性（AHR）、支气管肺泡灌洗液（BALF）中免疫细胞分布及血清中特异性细胞因子白细胞介素17 A（IL-17 A）和IL-4。还通过苏木精和伊红染色进行炎症的组织学检查以及中性粒细胞（NEU）、嗜酸性粒细胞（EOS）、IL-17 A和IL-4的免疫组织化学评估。与对照组相比，HDM/OVA/LPS致敏1-4组BALF细胞数、血清IL-17 A、IL-4、炎性细胞浸润、EOS、IL-17 A、IL-4均显著增加（P均<0.05）。HDM/OVA/LPS致敏的4组小鼠中，第4组的AHR、BALF中的细胞总数、NEU和EOS以及NEU和NEU/EOS比值均显著高于第1-3组（均P<0.05）。总之，100 µg HDM + 100 µg OVA + 15 µg LPS致敏成功建立了以嗜中性粒细胞炎性表型为主的重度哮喘模型。
Neutrophilic asthma (NA) is associated with a severe disease course and poor response to corticosteroids. The present study aimed to compare the effects of various concentrations of house dust mite (HDM) allergens, ovalbumin (OVA), the major egg allergen, and lipopolysaccharide (LPS) in combination on the onset of severe NA. Female C57BL/6 mice were grouped according to a random number table and intranasally sensitized with HDM/LPS/OVA extracts on days 0, 1 and 2 of the study. In group 1, mice received 50 µg HDM + 50 µg OVA + 15 µg LPS, mice in group 2 received 50 µg HDM + 100 µg OVA + 15 µg LPS, mice in group 3 received 100 µg HDM + 50 µg OVA + 15 µg LPS and those in group 4 received 100 µg HDM + 100 µg OVA + 15 µg LPS, while mice in the control group received saline only. The mice were then challenged by OVA solution with atomized excitation on days 14, 15, 18, 19 and 20 for 30 min each. Ethology, airway hyperresponsiveness (AHR), immune cell distributions in bronchoalveolar lavage fluid (BALF), and specific cytokines interleukin 17A (IL-17A) and IL-4 in serum were assessed. Histological examination of inflammation by hematoxylin and eosin staining and immunohistochemical assessment of neutrophils (NEU), eosinophils (EOS), IL-17A and IL-4 were also performed. Compared with the control group, the HDM/OVA/LPS-sensitized groups 1–4 had markedly increased BALF cells, serum interleukin IL-17A and IL-4, inflammatory cell infiltration, EOS as well as IL-17A and IL-4 by immunohistochemical staining (all P<0.05). Among the four HDM/OVA/LPS-sensitized groups, mice of group 4 had higher AHR, a significantly higher total cell number, NEU and EOS in BALF as well as significantly higher NEU and NEU/EOS ratios according to immunohistochemical staining when compared to groups 1–3 (P<0.05 for all). In conclusion, sensitization with 100 µg HDM + 100 µg OVA + 15 µg LPS successfully established a severe asthma model with a predominantly neutrophilic inflammatory phenotype.
登录
查看更多内容
DOI：
10.1186/1710-1492-10-30
发表时间：
2014
期刊：
Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology
影响因子：
--
作者：
Ren Y;Ichinose T;He M;Song Y;Yoshida Y;Yoshida S;Nishikawa M;Takano H;Sun G;Shibamoto T
通讯作者：
Shibamoto T
DOI：
10.1084/jem.20061134
发表时间：
2007-08-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bandukwala HS;Clay BS;Tong J;Mody PD;Cannon JL;Shilling RA;Verbeek JS;Weinstock JV;Solway J;Sperling AI
通讯作者：
Sperling AI
影响因子：
14.2
作者：
Moore, Wendy C.;Hastie, Annette T.;Li, Xingnan;Li, Huashi;Busse, William W.;Jarjour, Nizar N.;Wenzel, Sally E.;Peters, Stephen P.;Meyers, Deborah A.;Bleecker, Eugene R.
通讯作者：
Bleecker, Eugene R.
影响因子：
14.2
作者：
Celedon, Juan C.;Milton, Donald K.;Gold, Diane R.
通讯作者：
Gold, Diane R.
DOI：
10.12659/msm.881759
发表时间：
2011-05
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Lan F;Liu K;Zhang J;Qi Y;Li K;Lin P
通讯作者：
Lin P