Lipidomics profiling reveals the role of glycerophospholipid metabolism in psoriasis.
Lipidomics profiling reveals the role of glycerophospholipid metabolism in psoriasis.
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脂质组学分析揭示了甘油磷脂代谢在银屑病中的作用。
DOI：
10.1093/gigascience/gix087
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发表时间：
2017-10-01
期刊：
影响因子：
9.2
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Zeng C;Wen B;Hou G;Lei L;Mei Z;Jia X;Chen X;Zhu W;Li J;Kuang Y;Zeng W;Su J;Liu S;Peng C;Chen X
牛皮癣是一种常见的慢性炎症性皮肤病，因基因与环境的相互作用而变得复杂。尽管已经进行了基因组、转录组和蛋白质组分析来研究银屑病的发病机制，但代谢物，特别是脂质在银屑病中的作用仍不清楚。脂质不仅构成细胞膜双层的主体，而且还调节多种生物过程，例如细胞增殖、细胞凋亡、免疫、血管生成和炎症。在本研究中，采用非靶向脂质组学方法研究银屑病的脂质谱，并通过超高效液相色谱-串联四极杆质谱法鉴定银屑病的脂质代谢特征。收集并分析了 90 名参与者（45 名健康人和 45 名银屑病患者）的血浆样本。应用统计分析来发现疾病组和健康组之间的不同代谢物。此外，还进行了酶联免疫吸附测定来验证银屑病患者血浆中差异表达的脂质。最后，我们鉴定了几种脂质的差异表达，包括溶血磷脂酸（LPA）、溶血磷脂酰胆碱（LysoPC）、磷脂酰肌醇（PI）、磷脂酰胆碱（PC）和磷脂酸（PA）；在这些代谢物中，银屑病患者的 LPA、LysoPC 和 PA 显着升高，而 PC 和 PI 下调。我们发现银屑病患者血浆中甘油磷脂代谢的元素，如 LPA、LysoPC、PA、PI 和 PC 发生显着改变；这项研究描述了银屑病患者循环脂质的特征，并为脂质在银屑病中的作用提供了新的见解。
Psoriasis is a common and chronic inflammatory skin disease that is complicated by gene–environment interactions. Although genomic, transcriptomic, and proteomic analyses have been performed to investigate the pathogenesis of psoriasis, the role of metabolites in psoriasis, particularly of lipids, remains unclear. Lipids not only comprise the bulk of the cellular membrane bilayers but also regulate a variety of biological processes such as cell proliferation, apoptosis, immunity, angiogenesis, and inflammation. In this study, an untargeted lipidomics approach was used to study the lipid profiles in psoriasis and to identify lipid metabolite signatures for psoriasis through ultra-performance liquid chromatography-tandem quadrupole mass spectrometry. Plasma samples from 90 participants (45 healthy and 45 psoriasis patients) were collected and analyzed. Statistical analysis was applied to find different metabolites between the disease and healthy groups. In addition, enzyme-linked immunosorbent assay was performed to validate differentially expressed lipids in psoriatic patient plasma. Finally, we identified differential expression of several lipids including lysophosphatidic acid (LPA), lysophosphatidylcholine (LysoPC), phosphatidylinositol (PI), phosphatidylcholine (PC), and phosphatidic acid (PA); among these metabolites, LPA, LysoPC, and PA were significantly increased, while PC and PI were down-regulated in psoriasis patients. We found that elements of glycerophospholipid metabolism such as LPA, LysoPC, PA, PI, and PC were significantly altered in the plasma of psoriatic patients; this study characterizes the circulating lipids in psoriatic patients and provides novel insight into the role of lipids in psoriasis.
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DOI：
10.1016/j.bbalip.2014.09.004
发表时间：
2015-01-01
影响因子：
4.8
作者：
Elferink, Ronald P. J. Oude;Bolier, Ruth;Beuers, Ulrich H.
通讯作者：
Beuers, Ulrich H.
DOI：
10.1016/j.bbamcr.2007.11.014
发表时间：
2008-02-01
影响因子：
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影响因子：
5.4
作者：
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影响因子：
2.4
作者：
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通讯作者：
Rayens, W
影响因子：
4
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