Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate.
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate.
复制标题
正常、癌前和肿瘤犬前列腺中c-KIT和Ki 67表达的研究。
DOI：
10.1186/s12917-017-1304-0
复制
发表时间：
2017-12-06
影响因子：
2.6
通讯作者：
Laufer-Amorim R
中科院分区：
文献类型：
--
作者：
Fonseca-Alves CE;Kobayashi PE;Palmieri C;Laufer-Amorim R
c-KIT表达与人类前列腺癌骨转移有关，但c-KIT表达是否可以在犬前列腺组织中进行类似分类尚不清楚。本研究评估了c-KIT和Ki67在犬前列腺癌（PC）中的表达。采用免疫组化、RT-qPCR和western blot方法检测44例犬前列腺组织中c-KIT基因、蛋白和Ki67的表达。此外，我们还通过免疫印迹法研究了c-KIT蛋白在两种犬原发性前列腺癌细胞系中的表达。分析了11例正常前列腺、12例增殖性炎性萎缩（PIA）前列腺、18例前列腺癌、3例转移性病变和2例前列腺癌细胞培养（PC1和PC2）。前列腺组织呈现不同程度的膜性、细胞质或膜性/细胞质c-KIT染色。正常前列腺4例、PIA 4例、前列腺癌5例c-KIT阳性表达。转移灶中未见c-KIT免疫表达。与正常样本相比，犬前列腺癌和PIA样本中含有更多的ki67阳性细胞。c-KIT在正常、PIA、前列腺癌及转移癌中表达的中位相对定量（RQ）分别为0.6（0.1-2.5）、0.7（0.09-2.1）、0.7（0.09-5.1）和0.1（0.07-0.6）。在前列腺癌样本中，ki67阳性细胞数量与c-KIT转录物水平呈正相关。在细胞系中，PC1 c-KIT蛋白表达为阴性，PC2弱阳性。本研究发现c-KIT表达与增殖指数有很强的相关性，提示c-KIT可能影响细胞增殖。因此，c-KIT异质蛋白在样本（5例阳性和13例阴性前列腺癌样本）中的表达表明犬前列腺癌的个性化方法。本文的在线版本（10.1186/s12917-017-1304-0）包含补充内容，仅供授权用户使用。
c-KIT expression has been related to bone metastasis in human prostate cancer, but whether c-KIT expression can be similarly classified in canine prostatic tissue is unknown. This study assessed c-KIT and Ki67 expression in canine prostate cancer (PC). c-KIT gene and protein expression and Ki67 expression were evaluated in forty-four canine prostatic tissues by immunohistochemistry, RT-qPCR and western blot. Additionally, we have investigated c-KIT protein expression by immunoblotting in two primary canine prostate cancer cell lines. Eleven normal prostates, 12 proliferative inflammatory atrophy (PIA) prostates, 18 PC, 3 metastatic lesions and two prostate cancer cell cultures (PC1 and PC2) were analysed. The prostatic tissue exhibited varying degrees of membranous, cytoplasmic or membranous/cytoplasmic c-KIT staining. Four normal prostates, 4 PIA and 5 prostatic carcinomas showed positive c-KIT expression. No c-KIT immunoexpression was observed in metastases. Canine prostate cancer and PIA samples contained a higher number of Ki67-positive cells compared to normal samples. The median relative quantification (RQ) for c-KIT expression in normal, PIA and prostate cancer and metastatic samples were 0.6 (0.1-2.5), 0.7 (0.09-2.1), 0.7 (0.09-5.1) and 0.1 (0.07-0.6), respectively. A positive correlation between the number of Ki67-positive cells and c-KIT transcript levels was observed in prostate cancer samples. In the cell line, PC1 was negative for c-KIT protein expression, while PC2 was weakly positive. The present study identified a strong correlation between c-KIT expression and proliferative index, suggesting that c-KIT may influence cell proliferation. Therefore, c-KIT heterogeneous protein expression among the samples (five positive and thirteen negative prostate cancer samples) indicates a personalized approach for canine prostate cancer. The online version of this article (10.1186/s12917-017-1304-0) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
2.8
作者：
Lai, Chen-Li;van den Ham, Rene;Teske, Erik
通讯作者：
Teske, Erik
影响因子：
11.5
作者：
Hay, RV;Cao, B;Vande Woude, GF
通讯作者：
Vande Woude, GF
影响因子：
2.1
作者：
Chon, E.;McCartan, L.;Vail, D. M.
通讯作者：
Vail, D. M.
影响因子：
2.4
作者：
Palmieri, Chiara;Grieco, Valeria
通讯作者：
Grieco, Valeria
影响因子：
2.1
作者：
Sorenmo, K U;Goldschmidt, M H;Ferracone, J
通讯作者：
Ferracone, J