Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in Daucus carota Taproots in Response to Increased Salinity
Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in Daucus carota Taproots in Response to Increased Salinity
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胡萝卜直根中类胡萝卜素浓度和类胡萝卜素生物合成基因表达对盐度增加的响应变化
DOI：
10.3390/horticulturae8070650
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发表时间：
2022-07
期刊：
影响因子：
3.1
通讯作者：
Xiong AS
中科院分区：
文献类型：
--
作者：
Zhao YH;Deng YJ;Wang YH;Lou YR;He LF;Liu H;Li T;Yan ZM;Zhuang J;Xiong AS
研究盐处理下胡萝卜主根中类胡萝卜素的变化有助于探讨胡萝卜对盐胁迫的响应机制。以两个不同主根颜色的胡萝卜品种为材料，测定了其类胡萝卜素含量和10个类胡萝卜素相关基因的表达谱。盐胁迫下，‘KRD’和‘BHJS’主根中类胡萝卜素的生物合成被激活。RT-qPCR结果表明，盐胁迫下，“KRD”和“BHJS”中DcPSY 1、DcPSY 2、DcZDS 1、DcCRT 1和DcCRT 2基因的表达量均显著增加，而DcCHXE基因表达量下降，DcPDS基因表达量维持在基础水平。在‘KRD’主根中，DcLCYB、DcLCYE和DcCHXB 1的表达量显著上升。而“BHJS”主根的变化不明显。研究表明，盐胁迫可以刺激类胡萝卜素的生物合成。“KRD”和“BHJS”主根中叶黄素的积累可能主要归因于DcLCYE和DcCHXB 1转录本的变异。β-胡萝卜素积累的增加被推测为增加耐盐性。
Studying the changes of carotenoids in the taproot of carrots under salt treatment is helpful to probe the salt stress response mechanism of carrots. The carotenoid concentration and the expression profiles of 10 carotenoid-related genes were determined in two carrot cultivars with different taproot colors. Under salt stress, the biosynthesis of carotenoids in the taproot of both ‘KRD’ and ‘BHJS’ was activated. RT-qPCR manifested that the expression levels of DcPSY1, DcPSY2, DcZDS1, DcCRT1 and DcCRT2 increased significantly in both ‘KRD’ and BHJS’ under salt stress, but DcCHXE transcripts decreased and DcPDS transcripts maintained a basal level compared to that of the control. In the taproot of ‘KRD’, the expression level of DcLCYB, DcLCYE and DcCHXB1 climbed dramatically. However, there was no significant change in the taproot of ‘BHJS’. The study showed that salt stress can stimulate the biosynthesis of carotenoids. The accumulation of lutein in the taproots of ‘KRD’ and ‘BHJS’ may be mainly attributed to the variation in DcLCYE and DcCHXB1 transcripts. The increase in β-carotene accumulation is speculated to increase salt tolerance.
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影响因子：
3
作者：
Iorizzo, Massimo;Senalik, Douglas A.;Simon, Philipp W.
通讯作者：
Simon, Philipp W.
影响因子：
5.6
作者：
Shi Y;Guo J;Zhang W;Jin L;Liu P;Chen X;Li F;Wei P;Li Z;Li W;Wei C;Zheng Q;Chen Q;Zhang J;Lin F;Qu L;Snyder JH;Wang R
通讯作者：
Wang R
影响因子：
64.5
作者：
Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
5.1
作者：
Fuentes, Paulina;Pizarro, Lorena;Stange, Claudia
通讯作者：
Stange, Claudia
影响因子：
4.3
作者：
Frey, Anne;Boutin, Jean-Pierre;Marion-Poll, Annie
通讯作者：
Marion-Poll, Annie