The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters
The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters
复制标题
使用胚胎移植导管细胞分析子宫内膜容受性的可能性
DOI：
10.1007/s43032-021-00645-9
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发表时间：
2021
影响因子：
2.9
通讯作者：
Narahara Hisashi
中科院分区：
文献类型：
--
作者：
Goto Kaori;Kawano Yasushi;Utsunomiya Takafumi;Narahara Hisashi
研究子宫内膜受体标记物在着床过程中的表达具有重要意义。因此，我们研究是否有可能使用胚胎移植导管的细胞来分析子宫内膜容受性。本研究共入组了81例知情同意患者的81个周期。切割胚胎移植（ET）导管的头端并浸入专用试剂中。使用巴氏染色和免疫细胞化学进行细胞分布的确认。通过免疫细胞化学进行蛋白表达。采用定量逆转录-聚合酶链反应（RT-PCR）分析雌激素受体α（ER α）、孕激素受体（PR α）和同源框A10（homeobox A10）mRNA的表达。我们分析了子宫内膜细胞中与妊娠相关的基因表达谱之间的关系。从ET导管采集的样本显示子宫内膜细胞染色清晰。细胞主要为子宫内膜上皮细胞。未观察到宫颈细胞。蛋白表达也得到了证实。分析了三个与子宫内膜容受性相关的基因。与妊娠组相比，未妊娠组患者中的孕激素受体表达高1.4倍（p<0.05），同源框A10高2.8倍（p<0.01）。雌激素受体α表达在非妊娠组中倾向于更高（p=0.18）。我们的研究结果表明，子宫内膜容受性可以使用从ET导管获得的细胞进行评估。通过比较ET时接受性和非接受性子宫内膜，这种方法可能有助于阐明着床失败的原因。
It is very important to investigate the expression of endometrial receptive markers in the endometrium during implantation. Therefore, we examined whether it would be possible to analyze endometrial receptivity using cells from embryo transfer catheters. A total of 81 cycles from 81 consenting patients were enrolled in this study. The tip of the embryo transfer (ET) catheter was cut and immersed in a dedicated reagent. Confirmation of cell distribution was carried out using a Papanicolaou stain and immunocytochemistry. Protein expression was carried out by immunocytochemistry. The expressions of estrogen receptor α, progesterone receptor, and homeobox A10 mRNA were analyzed using quantitative reverse transcription-polymerase chain reaction. We analyzed the relationship between the gene expression profiles associated with pregnancy from endometrial cells. Samples collected from the ET catheter showed clear staining for endometrial cells. Most of the cells were endometrial epithelial cells. Cervical cells were not observed. The protein expression was also confirmed. Three genes were analyzed that are associated with endometrial receptivity. Progesterone receptor expression was 1.4-fold (p<0.05) and homeobox A10 was 2.8-fold (p<0.01) higher in patients who became non-pregnant group, compared to the pregnant group. Estrogen receptor α expression tended to be higher in the non-pregnant group (p=0.18). Our results suggest that endometrial receptivity can be evaluated using cells obtained from the ET catheter. This method may be useful for elucidating the cause of implantation failure by comparing a receptive and non-receptive endometrium at the time of ET.
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