miRT: a database of validated transcription start sites of human microRNAs.
miRT: a database of validated transcription start sites of human microRNAs.
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DOI：
10.1016/j.gpb.2012.08.005
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发表时间：
2012-10
影响因子：
9.5
通讯作者：
Bandyopadhyay, Sanghamitra
中科院分区：
文献类型：
--
作者：
Bhattacharyya, Malay;Das, Manali;Bandyopadhyay, Sanghamitra
microRNAs（miRNAs）是一类长度约为22 nt的内源性非编码小分子RNA，在许多生物学过程中发挥着重要作用。这些短RNA通过与其3′-UTR结合或通过翻译抑制来调节mRNA的表达。目前的研究主要集中在成熟的miRNAs如何调控mRNAs上，然而，关于其转录位点的知识非常有限。已知初级miRNA（pri-miR）首先从DNA转录，随后通过内切核酸酶活性形成前体miRNA（pre-miR），其最终产生成熟的miRNA。到目前为止，许多pre-miRs和成熟miRNAs已被实验验证。但遗憾的是，pri-miR的位点、启动子和相关转录起始位点（TSS）的鉴定仍在进行中。已知的成熟miRNAs中只有约40%的人的TSS已被报道。这些信息虽然有限，但可能有助于进一步研究miRNAs的调控。在本文中，我们提供了一个新的数据库验证的miRNA TSS，miRT，通过收集数据，从几个实验研究，验证miRNA TSS，并可供完整下载。我们目前的miRT作为一个Web服务器，它也可以转换不同的基因组之间构建的TSS位点。miRT可能是miRNA调控高级研究的宝贵资源，可在http://www.isical.ac.in/~bioinfo_miu/miRT/miRT.php免费访问。
MicroRNAs (miRNAs) are small endogenous non-coding RNAs of about 22 nt in length that take crucial roles in many biological processes. These short RNAs regulate the expression of mRNAs by binding to their 3′-UTRs or by translational repression. Many of the current studies focus on how mature miRNAs regulate mRNAs, however, very limited knowledge is available regarding their transcriptional loci. It is known that primary miRNAs (pri-miRs) are first transcribed from the DNA, followed by the formation of precursor miRNAs (pre-miRs) by endonuclease activity, which finally produces the mature miRNAs. Till date, many of the pre-miRs and mature miRNAs have been experimentally verified. But unfortunately, identification of the loci of pri-miRs, promoters and associated transcription start sites (TSSs) are still in progress. TSSs of only about 40% of the known mature miRNAs in human have been reported. This information, albeit limited, may be useful for further study of the regulation of miRNAs. In this paper, we provide a novel database of validated miRNA TSSs, miRT, by collecting data from several experimental studies that validate miRNA TSSs and are available for full download. We present miRT as a web server and it is also possible to convert the TSS loci between different genome built. miRT might be a valuable resource for advanced research on miRNA regulation, which is freely accessible at: http://www.isical.ac.in/~bioinfo_miu/miRT/miRT.php.
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作者：
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