Aqueous Angiography with Fluorescein and Indocyanine Green in Bovine Eyes.
Aqueous Angiography with Fluorescein and Indocyanine Green in Bovine Eyes.
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DOI：
10.1167/tvst.5.6.5
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发表时间：
2016-11
影响因子：
3
通讯作者：
Weinreb RN
中科院分区：
文献类型：
--
作者：
Huang AS;Saraswathy S;Dastiridou A;Begian A;Legaspi H;Mohindroo C;Tan JC;Francis BA;Caprioli J;Hinton DR;Weinreb RN
我们的特点是水血管造影作为一个实时的房水流出成像（AHO）的方式在牛眼与两种示踪剂的不同分子特征。获得牛眼球摘除术（n = 31），并通过Lewicky AC保持器通过1 mm侧孔灌注平衡盐溶液。在10 mm Hg下分别或依次将黄绿素（2.5%）或吲哚菁绿色（ICG; 0.4%）引入前房内。与血管造影，红外和荧光图像采集。同时进行眼前节光学相干断层扫描（OCT），并将可固定的荧光葡聚糖引入眼内，对血管造影阳性和阴性区域进行组织学分析。牛眼荧光素和ICG的水血管造影产生了高质量的图像节段性模式。随着时间的推移，ICG保持了更好的腔内存在。血管造影阳性，但不是阴性，区域表现为前节OCT的巩膜内腔。荧光葡聚糖的水血管造影导致其在AHO通路中被捕获。在牛眼中，ICG随后荧光素的连续水相血管造影显示出类似的模式。模型牛眼的眼水血管造影显示节段性血管造影流出模式，荧光素或ICG作为示踪剂。通过房水血管造影进一步表征节段性AHO可能允许智能放置小梁旁路微创青光眼手术，以改善手术结果。
We characterize aqueous angiography as a real-time aqueous humor outflow imaging (AHO) modality in cow eyes with two tracers of different molecular characteristics. Cow enucleated eyes (n = 31) were obtained and perfused with balanced salt solution via a Lewicky AC maintainer through a 1-mm side-port. Fluorescein (2.5%) or indocyanine green (ICG; 0.4%) were introduced intracamerally at 10 mm Hg individually or sequentially. With an angiographer, infrared and fluorescent images were acquired. Concurrent anterior segment optical coherence tomography (OCT) was performed, and fixable fluorescent dextrans were introduced into the eye for histologic analysis of angiographically positive and negative areas. Aqueous angiography in cow eyes with fluorescein and ICG yielded high-quality images with segmental patterns. Over time, ICG maintained a better intraluminal presence. Angiographically positive, but not negative, areas demonstrated intrascleral lumens with anterior segment OCT. Aqueous angiography with fluorescent dextrans led to their trapping in AHO pathways. Sequential aqueous angiography with ICG followed by fluorescein in cow eyes demonstrated similar patterns. Aqueous angiography in model cow eyes demonstrated segmental angiographic outflow patterns with either fluorescein or ICG as a tracer. Further characterization of segmental AHO with aqueous angiography may allow for intelligent placement of trabecular bypass minimally invasive glaucoma surgeries for improved surgical results.
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DOI：
10.1196/annals.1430.028
发表时间：
2008-01-01
期刊：
FLUORESCENCE METHODS AND APPLICATIONS: SPECTROSCOPY, IMAGING, AND PROBES
影响因子：
--
作者：
Rodriguez, Hernan B.;Roman, Enrique San
通讯作者：
Roman, Enrique San
影响因子：
3.4
作者：
Lu, Zhaozeng;Overby, Darryl R.;Gong, Haiyan
通讯作者：
Gong, Haiyan
影响因子：
3.9
作者：
EMMART, EW
通讯作者：
EMMART, EW
流出阻力产生的不断变化的范式：朝向JCT和内壁内皮的协同模型。
影响因子：
3.4
作者：
Overby, Darryl R.;Stamer, W. Daniel;Johnson, Mark
通讯作者：
Johnson, Mark
影响因子：
3.9
作者：
Keane, P. A.;Sadda, S. R.
通讯作者：
Sadda, S. R.