Remote excitation of neuronal circuits using low-intensity, low-frequency ultrasound.
Remote excitation of neuronal circuits using low-intensity, low-frequency ultrasound.
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DOI：
10.1371/journal.pone.0003511
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Majestic C
中科院分区：
文献类型：
--
作者：
Tyler WJ;Tufail Y;Finsterwald M;Tauchmann ML;Olson EJ;Majestic C
拥有非侵入性地引发脑回路活动的能力产生了巨大的实验和治疗能力。大多数目前采用的神经刺激方法依赖于刺激电极或光子发射装置的某种侵入性使用。由于其能够以聚焦的方式无创地传播通过骨骼和其他组织，超声（US）的实施代表了当前神经调节策略的一种引人注目的替代方法。在这里，我们研究了低强度，低频超声（LILFU）对神经元活动的影响。通过海马切片培养和离体小鼠大脑传输US波形，我们确定LILFU能够远程和非侵入性地兴奋神经元和网络活动。我们的研究结果表明LILFU可以通过激活电压门控钠通道以及电压门控钙通道来刺激神经元的电活动。LILFU诱导的神经元活动变化足以触发SNARE介导的胞吐作用和海马回路中的突触传递。由于LILFU可以刺激神经元的电活动和钙信号以及中枢突触传递，我们得出结论，US提供了一个强大的工具，用于远程调节脑回路活动。
Possessing the ability to noninvasively elicit brain circuit activity yields immense experimental and therapeutic power. Most currently employed neurostimulation methods rely on the somewhat invasive use of stimulating electrodes or photon-emitting devices. Due to its ability to noninvasively propagate through bone and other tissues in a focused manner, the implementation of ultrasound (US) represents a compelling alternative approach to current neuromodulation strategies. Here, we investigated the influence of low-intensity, low-frequency ultrasound (LILFU) on neuronal activity. By transmitting US waveforms through hippocampal slice cultures and ex vivo mouse brains, we determined LILFU is capable of remotely and noninvasively exciting neurons and network activity. Our results illustrate that LILFU can stimulate electrical activity in neurons by activating voltage-gated sodium channels, as well as voltage-gated calcium channels. The LILFU-induced changes in neuronal activity were sufficient to trigger SNARE-mediated exocytosis and synaptic transmission in hippocampal circuits. Because LILFU can stimulate electrical activity and calcium signaling in neurons as well as central synaptic transmission we conclude US provides a powerful tool for remotely modulating brain circuit activity.
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影响因子：
4.8
作者：
Hsu, Horng-Chaung;Fong, Yi-Chin;Tang, Chih-Hsin
通讯作者：
Tang, Chih-Hsin
DOI：
10.1073/pnas.97.18.10242
发表时间：
2000-08-29
影响因子：
11.1
作者：
Jin, KL;Mao, XO;Greenberg, DA
通讯作者：
Greenberg, DA
影响因子：
--
作者：
Ebisawa, K;Hata, KI;Watanabe, H
通讯作者：
Watanabe, H
影响因子：
2.4
作者：
FRY, WJ;WULFF, VJ;FRY, FJ
通讯作者：
FRY, FJ
影响因子：
4.2
作者：
Clement, GT
通讯作者：
Clement, GT