Downregulation of CSD2 by a heat-inducible miR398 is required for thermotolerance in Arabidopsis

Downregulation of CSD2 by a heat-inducible miR398 is required for thermotolerance in Arabidopsis
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拟南芥耐热性需要热诱导型 miR398 下调 CSD2

DOI:
10.4161/psb.24952
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发表时间:
2013-05
期刊:
Plant Signaling & Behavior
影响因子:
--
通讯作者:
Jianhua Zhu.
Jianhua Zhu.
中科院分区:
其他
文献类型:
--
作者:
Xiaoyan Lu, Qingmei Guan;Jianhua Zhu.

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MicroRNAs(MiRNAs)在植物生长发育和非生物胁迫反应中发挥着重要作用。我们在这里报道了热应激迅速诱导miR398并减少其靶基因CSD2的转录。过量表达miR398抗性形式的CSD2的转基因植株比过度表达正常编码序列的CSD2的转基因植株对热胁迫更敏感。在过量表达miR398抗性形式的CSD2的热敏转基因植物中,热应激转录因子(HSFs)和热休克蛋白(HSPs)的表达减少。我们的结果表明,热诱导miR398下调CSD2是拟南芥耐热性所必需的。
MicroRNAs (miRNAs) play important roles in plant growth and development and abiotic stress responses. We report here that heat stress rapidly induces miR398 and reduces transcript of its target gene CSD2. Transgenic plants overexpressing the miR398-resistant form of CSD2 are more sensitive to heat stress than transgenic plants overexpressing normal coding sequence of CSD2. Expression of heat stress transcription factors (HSFs) and heat shock proteins (HSPs) is reduced in the heat-sensitive transgenic plants overexpressing miR398-resistant form of CSD2. Our results suggest that downregulation of CSD2 by the heat-inducible miR398 is required for thermotolerance in Arabidopsis.
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