RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast.
RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast.
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DOI：
10.1186/gb-2010-11-7-r75
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发表时间：
2010
期刊：
影响因子：
12.3
通讯作者：
Rando OJ
中科院分区：
文献类型：
--
作者：
Kim TS;Liu CL;Yassour M;Holik J;Friedman N;Buratowski S;Rando OJ
通过微阵列和深度测序进行的全基因组RNA丰度分析的使用激发了我们对转录控制的理解的革命。然而，mRNA丰度的变化反映了RNA产生、加工和降解变化的综合效应，因此，mRNA水平提供了转录调控的封闭视图。为了部分解开这些问题，我们进行了全基因组RNA聚合酶II（PolII）定位分析在芽殖酵母在两个不同的应激反应时间过程。虽然mRNA的变化在很大程度上反映了转录的变化，但mRNA水平的变化仍然很大，这不是PolII丰度变化的原因。我们发现，每个PolII表现出“过量”的mRNA产生的基因是丰富的那些重叠的神秘的转录本，表明一个普遍的作用，非生产性或调节转录控制基因表达。最后，我们的特点PolII本地化的变化时，PolII基因失活的rpb 1 -1温度敏感性突变。我们发现，PolII在限制性温度下大约一小时后从染色质中丢失，并且不同位点的PolII丢失率存在很大的差异。总之，这些结果提供了一个全球性的观点之间的关系PolII和mRNA的生产芽殖酵母。
The use of genome-wide RNA abundance profiling by microarrays and deep sequencing has spurred a revolution in our understanding of transcriptional control. However, changes in mRNA abundance reflect the combined effect of changes in RNA production, processing, and degradation, and thus, mRNA levels provide an occluded view of transcriptional regulation. To partially disentangle these issues, we carry out genome-wide RNA polymerase II (PolII) localization profiling in budding yeast in two different stress response time courses. While mRNA changes largely reflect changes in transcription, there remains a great deal of variation in mRNA levels that is not accounted for by changes in PolII abundance. We find that genes exhibiting 'excess' mRNA produced per PolII are enriched for those with overlapping cryptic transcripts, indicating a pervasive role for nonproductive or regulatory transcription in control of gene expression. Finally, we characterize changes in PolII localization when PolII is genetically inactivated using the rpb1-1 temperature-sensitive mutation. We find that PolII is lost from chromatin after roughly an hour at the restrictive temperature, and that there is a great deal of variability in the rate of PolII loss at different loci. Together, these results provide a global perspective on the relationship between PolII and mRNA production in budding yeast.
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影响因子：
5.3
作者：
GILMOUR, DS;LIS, JT
通讯作者：
LIS, JT
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作者：
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Becskei, Attila
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作者：
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