Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis.
Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis.
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DOI：
10.1186/s12866-024-03201-y
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发表时间：
2024-02-01
期刊：
影响因子：
4.2
通讯作者：
Grant, Andrew J.
中科院分区：
文献类型：
--
作者：
Stoakes, Emily;Chen, Xuanlin;Kalmar, Lajos;Baker, Dave;Evans, Rhiannon;Rudder, Steven;Grant, Andrew J.
空肠弯曲菌和结肠弯曲菌是世界范围内细菌性胃肠炎的主要致病菌，是已知的专性微嗜氧菌。尽管这两个物种对氧气及其还原产物敏感，但它们很容易从保存在大气条件下的动物食品中分离出来，因为它们面临着高氧气压力水平。本研究利用转座子定向插入位点测序技术(Tradis)研究了一株空肠弯曲菌和两株大肠埃希氏菌利用过氧化氢模拟氧化应激来克服氧化应激的能力。研究人员确定了每个菌株都需要的抗氧化应激基因，但也对三个菌株进行了比较。Perr和ahpC基因的突变被发现提高了弯曲杆菌对过氧化氢的耐受性。这些蛋白在氧化应激中的作用在空肠弯曲菌中是已知的，但这一数据表明，它们很可能在大肠杆菌中发挥类似的作用。CzcD突变降低了弯曲菌对H_2O_2的耐受性。作为锌的出口商，它的作用以前并没有与氧化应激联系在一起。使用大肠杆菌15-537360中定义的perR和czcD缺失来确认TRADIS数据。这是第一次研究空肠弯曲菌和大肠埃希氏菌在氧化应激条件下的基因适合性，强调了这两个物种某些基因的相似作用，并强调了在氧化应激下发挥作用的其他基因。网上版载有补充材料，可在10.1186/s12866-024-03201-y查阅。
Campylobacter jejuni and Campylobacter coli are the major causative agents of bacterial gastroenteritis worldwide and are known obligate microaerophiles. Despite being sensitive to oxygen and its reduction products, both species are readily isolated from animal food products kept under atmospheric conditions where they face high oxygen tension levels. In this study, Transposon Directed Insertion-site Sequencing (TraDIS) was used to investigate the ability of one C. jejuni strain and two C. coli strains to overcome oxidative stress, using H2O2 to mimic oxidative stress. Genes were identified that were required for oxidative stress resistance for each individual strain but also allowed a comparison across the three strains. Mutations in the perR and ahpC genes were found to increase Campylobacter tolerance to H2O2. The roles of these proteins in oxidative stress were previously known in C. jejuni, but this data indicates that they most likely play a similar role in C. coli. Mutation of czcD decreased Campylobacter tolerance to H2O2. The role of CzcD, which functions as a zinc exporter, has not previously been linked to oxidative stress. The TraDIS data was confirmed using defined deletions of perR and czcD in C. coli 15-537360. This is the first study to investigate gene fitness in both C. jejuni and C. coli under oxidative stress conditions and highlights both similar roles for certain genes for both species and highlights other genes that have a role under oxidative stress. The online version contains supplementary material available at 10.1186/s12866-024-03201-y.
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影响因子：
5.2
作者：
Rodrigues RC;Haddad N;Chevret D;Cappelier JM;Tresse O
通讯作者：
Tresse O
弯曲杆菌大肠杆菌临床分离株的完整基因组序列15-537360。
DOI：
10.1128/genomea.01056-13
发表时间：
2013-12-12
期刊：
Genome announcements
影响因子：
--
作者：
Pearson BM;Rokney A;Crossman LC;Miller WG;Wain J;van Vliet AH
通讯作者：
van Vliet AH
影响因子：
5.2
作者：
Gundogdu O;da Silva DT;Mohammad B;Elmi A;Mills DC;Wren BW;Dorrell N
通讯作者：
Dorrell N
影响因子：
4
作者：
Lambert, RJW;Pearson, J
通讯作者：
Pearson, J
影响因子：
3.2
作者：
Gardner, Susan P.;Olson, Jonathan W.
通讯作者：
Olson, Jonathan W.