Comparison of Proteomics Profiles of Campylobacter jejuni Strain Bf under Microaerobic and Aerobic Conditions.
Comparison of Proteomics Profiles of Campylobacter jejuni Strain Bf under Microaerobic and Aerobic Conditions.
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DOI：
10.3389/fmicb.2016.01596
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发表时间：
2016
影响因子：
5.2
通讯作者：
Tresse O
中科院分区：
文献类型：
--
作者：
Rodrigues RC;Haddad N;Chevret D;Cappelier JM;Tresse O
空肠弯曲菌是人类食源性细菌性肠炎的主要原因之一。尽管被认为是专性微好氧微生物，C。空肠经常暴露于氧化应激。然而，其在传播给人类期间在大气氧水平下生存的适应性策略仍不清楚。最近，临床上C.空肠菌株Bf因其在环境大气下出乎意料的生长能力而被挑选出来。在这里，我们的目标是利用二维蛋白质电泳、基因表达和酶活性更好地了解其非典型耐氧特性的生物学机制。在微氧和有氧条件下培养的47种蛋白质被鉴定出具有显著不同的丰度。与微氧条件相比，有氧条件下过表达的蛋白质主要属于氧化应激反应、三羧酸循环酶、铁吸收和调节以及氨基酸吸收。与微氧条件相比，有氧条件下与氧化应激相关的蛋白质的较高丰度与相应编码基因的显著较高的转录水平相关。此外，在菌株Bf中观察到更高的过氧化氢酶当量活性。尽管C.空肠，这项研究揭示了菌株Bf配备承受氧化应激。这种能力可能有助于特定菌株的出现和持续存在。空肠在整个食品加工或巨噬细胞攻击在人类感染。
Campylobacter jejuni accounts for one of the leading causes of foodborne bacterial enteritis in humans. Despite being considered an obligate microaerobic microorganism, C. jejuni is regularly exposed to oxidative stress. However, its adaptive strategies to survive the atmospheric oxygen level during transmission to humans remain unclear. Recently, the clinical C. jejuni strain Bf was singled out for its unexpected ability to grow under ambient atmosphere. Here, we aimed to understand better the biological mechanisms underlying its atypical aerotolerance trait using two-dimensional protein electrophoresis, gene expression, and enzymatic activities. Forty-seven proteins were identified with a significantly different abundance between cultivation under microaerobic and aerobic conditions. The over-expressed proteins in aerobiosis belonged mainly to the oxidative stress response, enzymes of the tricarboxylic acid cycle, iron uptake, and regulation, and amino acid uptake when compared to microaerobic conditions. The higher abundance of proteins related to oxidative stress was correlated to dramatically higher transcript levels of the corresponding encoding genes in aerobic conditions compared to microaerobic conditions. In addition, a higher catalase-equivalent activity in strain Bf was observed. Despite the restricted catabolic capacities of C. jejuni, this study reveals that strain Bf is equipped to withstand oxidative stress. This ability could contribute to emergence and persistence of particular strains of C. jejuni throughout food processing or macrophage attack during human infection.
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影响因子：
3
作者：
JONES, DM;SUTCLIFFE, EM;CURRY, A
通讯作者：
CURRY, A
DOI：
10.3390/ijerph10126292
发表时间：
2013-11-26
影响因子：
--
作者：
Epps SV;Harvey RB;Hume ME;Phillips TD;Anderson RC;Nisbet DJ
通讯作者：
Nisbet DJ
影响因子：
5.1
作者：
Kendall JJ;Barrero-Tobon AM;Hendrixson DR;Kelly DJ
通讯作者：
Kelly DJ
影响因子：
2.8
作者：
He, Yiping;Frye, Jonathan G.;Chen, Chin-Yi
通讯作者：
Chen, Chin-Yi
影响因子：
3.2
作者：
Baillon, MLA;van Vliet, AHM;Penn, CW
通讯作者：
Penn, CW