Endogenous distal airway progenitor cells, lung mechanics, and disproportionate lobar growth following long-term postpneumonectomy in mice.
Endogenous distal airway progenitor cells, lung mechanics, and disproportionate lobar growth following long-term postpneumonectomy in mice.
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DOI：
10.1002/stem.1377
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发表时间：
2013-07
期刊：
影响因子：
5.2
通讯作者：
Weiss, Daniel J.
中科院分区：
文献类型：
--
作者：
Eisenhauer, Philip;Earle, Benjamin;Loi, Roberto;Sueblinvong, Viranuj;Goodwin, Meagan;Allen, Gilman B.;Lundblad, Lennart;Mazan, Melissa R.;Hoffman, Andrew M.;Weiss, Daniel J.
利用小鼠肺切除术后（PNY）代偿性肺生长的模型，我们先前观察到肺切除术后3、7和14天时假定的内源性远端气道祖细胞群（位于支气管肺泡管连接处（BADJ）的CCSPpos/pro-SPCpos细胞）数量增加，在PNY后28天时恢复至基线。由于对这些细胞的起源知之甚少，我们评估了骨髓细胞是否有助于这些或其他细胞在长期的PNY后肺再生过程中的池。幼稚和性别不匹配的嵌合体小鼠接受左侧PNY，并在1、2和3个月时评价BADJ CCSPpos/pro-SPCpos细胞的数量和移植为气道或肺泡上皮的供体来源的骨髓细胞的存在。还在PNY后12个月检查了非嵌合小鼠的BADJ CCSPpos/pro-SPCpos细胞数量。值得注意的是，右副叶（RAL）在12个月内继续不成比例地生长，这是一个以前没有描述过的新发现。肺力学评估显示PNY后肺硬度增加，在一年内显著降低，但相对于1岁的幼稚对照组仍升高。然而，PNY后≥1个月的CCSPpos/pro-SPCpos BADJ细胞数量与未处理对照组相当，即使在RAL持续生长12个月后也是如此。值得注意的是，在PNY后3个月内，没有供体骨髓来源的细胞作为气道或肺泡上皮细胞移植，包括BADJ处的细胞。这些研究表明，肺上皮细胞，包括CCSPpos/pro-SPCpos细胞，在小鼠PNY后肺生长期间没有从骨髓来源的细胞中补充。
Utilizing a model of post-pneumonectomy (PNY) compensatory lung growth in mice, we previously observed an increase in numbers of a putative endogenous distal airway progenitor cell population (CCSPpos/pro-SPCpos cells located at bronchoalveolar duct junctions (BADJ)), at 3, 7, and 14 days after pneumonectomy, returning to baseline at 28 days post-PNY. As the origin of these cells is poorly understood, we evaluated whether bone marrow cells contributed to the pool of these or other cells during prolonged post-PNY lung regrowth. Naïve and sex-mismatched chimeric mice underwent left PNY and were evaluated at 1, 2 and 3 months for numbers of BADJ CCSPpos/pro-SPCpos cells and presence of donor-derived marrow cells engrafted as airway or alveolar epithelium. Non-chimeric mice were also examined at 12 months after PNY for numbers of BADJ CCSPpos/pro-SPCpos cells. Notably, the right accessory lobe (RAL) continued to grow disproportionately over 12 months, a novel finding not previously described. Assessment of lung mechanics demonstrated an increase in lung stiffness following PNY, which significantly diminished over one year, but remained elevated relative to 1 year-old naïve controls. However, the number of CCSPpos/pro-SPCpos BADJ cells ≥1 month following PNY was equivalent to that found in naïve controls even after 12 months of continued RAL growth. Notably, no donor bone marrow-derived cells engrafted as airway or alveolar epithelial cells, including those at the BADJ, up to 3 months after PNY. These studies suggest that lung epithelial cells, including CCSPpos/pro-SPCpos cells, are not replenished from marrow-derived cells during post-PNY lung growth in mice.
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影响因子：
64.5
作者：
Kumar PA;Hu Y;Yamamoto Y;Hoe NB;Wei TS;Mu D;Sun Y;Joo LS;Dagher R;Zielonka EM;Wang de Y;Lim B;Chow VT;Crum CP;Xian W;McKeon F
通讯作者：
McKeon F
影响因子：
5.2
作者：
Kassmer, Susannah H.;Bruscia, Emanuela M.;Zhang, Ping-Xia;Krause, Diane S.
通讯作者：
Krause, Diane S.
影响因子：
3.3
作者：
Allen, GB;Pavone, LA;Nieman, GF
通讯作者：
Nieman, GF
影响因子：
12.4
作者：
Duchesneau, Pascal;Wong, Amy P.;Waddell, Thomas K.
通讯作者：
Waddell, Thomas K.
DOI：
10.3109/00365519209115516
发表时间：
1992-11-01
影响因子：
2.1
作者：
KUBOI, S;MIZUUCHI, A;KIDA, K
通讯作者：
KIDA, K