Estrogen receptor (ER)alpha, but not ERbeta, gene is expressed in growth hormone-releasing hormone neurons of the male rat hypothalamus.
Estrogen receptor (ER)alpha, but not ERbeta, gene is expressed in growth hormone-releasing hormone neurons of the male rat hypothalamus.
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雌激素受体 (ER)α（而非 ERβ）基因在雄性大鼠下丘脑的生长激素释放激素神经元中表达。
DOI：
10.1210/endo.142.2.7933
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发表时间：
2001
期刊：
影响因子：
4.8
通讯作者：
I. Wakabayashi
中科院分区：
文献类型：
--
作者：
J. Kamegai;H. Tamura;Takako Shimizu;S. Ishii;H. Sugihara;I. Wakabayashi
垂体生长激素的合成和释放受下丘脑神经肽、生长激素释放激素（GHRH）和生长抑素（SS）的相反作用控制。大鼠生长激素分泌方式存在明显的性别差异。早期的报告表明，性腺类固醇在新生儿期有重要的印记作用。最近，我们的实验室和其他人报道，生长激素分泌模式改变短期性腺类固醇治疗成年大鼠，这表明性腺类固醇激素也是重要的决定因素的生长激素分泌模式在成年生活。然而，性腺类固醇激素在成年大鼠下丘脑中的作用部位仍不清楚。在这项研究中，我们使用原位杂交在成年雄性大鼠的大脑，以确定是否GHRH神经元和/或SS神经元共表达雌激素受体α（ER α）和ER β基因。在成年雄性大鼠下丘脑内侧基底部，ER α mRNA主要分布于内侧视前区（MPA）和弓状核（ARC），ER β mRNA主要分布于MPA、视上核和室旁核。从使用相邻切片的研究来看，含ER α mRNA的细胞的分布似乎与仅在ARC中表达GHRH和SS的细胞的分布部分重叠。另一方面，含ER β mRNA的细胞的分布似乎与GHRH细胞或SS细胞不重叠。双标记原位杂交研究表明，在ARC中，70%的GHRH神经元含有ER α mRNA，而不到5%的SS神经元表达ER α基因。这些结果表明，GHRH神经元是雌激素的直接靶细胞，雌激素可能通过ER α直接作用于GHRH神经元，从而改变GH的分泌模式。
GH synthesis and release from pituitary somatotropes is controlled by the opposing actions of the hypothalamic neuropeptides, GH-releasing hormone (GHRH), and somatostatin (SS). There is a striking sex difference in the pattern of GH secretion in rats. Early reports indicate that gonadal steroids have important imprinting effects during the neonatal period. Recently, our laboratory and others have reported that the GH secretory pattern is altered by short-term gonadal steroid treatment in adult rat, suggesting that gonadal steroids are also important determinants of the pattern of GH secretion during adult life. However, the site of action of gonadal steroids in the adult rat hypothalamus is still unknown. In this study, we used in situ hybridization in the adult male rat brain to determine whether GHRH neurons and/or SS neurons coexpress estrogen receptor alpha (ERalpha) and ERss genes. In the medial basal hypothalamus of adult male rat, the ERalpha messenger RNA (mRNA) was located in medial preoptic area (MPA) and arcuate nucleus (ARC), whereas ERss mRNA was detected in MPA, supraoptic nucleus, and paraventricular nucleus. From studies using adjacent sections, the distribution of ERalpha mRNA-containing cells appeared to overlap in part with those of GHRH and SS expressing cells only in the ARC. On the other hand, the distribution of ERss mRNA-containing cells does not appear to overlap with GHRH cells or SS cells. The double label in situ hybridization studies showed that in the ARC, 70% of GHRH neurons contain ERalpha mRNA, whereas less than 5% of SS neurons expressed the ERalpha gene. These results indicated that GHRH neurons are direct target cells for estrogens, and estrogens may act directly on GHRH neurons through ERalpha during adult life to modify GH secretory patterns.
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雌二醇对大鼠下丘脑生长激素释放因子（GRF）直接作用的证据：[3H]雌二醇在 GRF 神经元中的定位。
影响因子：
4.8
作者：
Shirasu,K;Stumpf,WE;Sar,M
通讯作者：
Sar,M
生长激素分泌素和生长抑素在调节生长激素分泌中的生理作用。
DOI：
10.1016/0006-291x(82)91758-2
发表时间：
1982
影响因子：
3.1
作者：
Wehrenberg,WB;Ling,N;Böhlen,P;Esch,F;Brazeau,P;Guillemin,R
通讯作者：
Guillemin,R
成年大鼠中生长激素释放激素基因的性别二态性表达不是由循环性腺激素介导的。
影响因子：
4.8
作者：
Maiter,D;Koenig,JI;Kaplan,LM
通讯作者：
Kaplan,LM
调节大鼠生长激素分泌的肽的性别和发育差异。
影响因子：
4.1
作者：
Gabriel,SM;Millard,WJ;Koenig,JI;Badger,TM;Russell,WE;Maiter,DM;Martin,JB
通讯作者：
Martin,JB
成人短期暴露于雌二醇会使大鼠自发和生长激素释放因子刺激的生长激素分泌的男性模式女性化。
影响因子：
4.8
作者：
Painson,JC;Thorner,MO;Krieg,RJ;Tannenbaum,GS
通讯作者：
Tannenbaum,GS