A missense mutation of plastid RPS4 is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis).
A missense mutation of plastid RPS4 is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis).
复制标题
质体 RPS4 的错义突变与大白菜 (Brassica Campestris ssp pekinensis) 叶绿素缺乏有关
DOI：
10.1186/s12870-018-1353-y
复制
发表时间：
2018-06-25
影响因子：
5.3
通讯作者：
Feng H
中科院分区：
文献类型：
--
作者：
Tang X;Wang Y;Zhang Y;Huang S;Liu Z;Fei D;Feng H
质体突变体是研究质体基因功能的理想材料。关于大麦和烟草中质体基因的功能已进行了大量的研究，但在大白菜中的相关研究较少。用甲基磺酸乙酯处理分离的小孢子，得到一个叶绿素缺乏的大白菜突变体，与野生型“福田50”（FT）相比，其内叶呈均匀的淡绿色，生长缓慢。遗传学分析表明cdm为细胞质遗传。生理和超微结构分析表明，cdm的光合作用受损，叶绿体发育异常。利用下一代测序技术，将cdm和'FT'的完整质体分别重新定位到大白菜的参考基因组上，发现了一个A-to-C碱基替换，突变率大于99%。质体核糖体蛋白S4的错义突变导致第193位残基的甘氨酸被缬氨酸取代。使用定量实时PCR分析rps 4的表达水平，发现在“FT”中比在“FT”中低。RNA凝胶印迹分析表明，成熟的23 S rRNA、16 S rRNA、5S rRNA和4.5S rRNA的丰度显著降低，23 S、16 S rRNA和4.5S rRNA的加工严重受损，影响了cdm中核糖体的功能。这些结果表明cdm是一个质体突变体，叶绿素缺乏可能是由于质体编码的rps 4的A到C碱基取代，损害了rRNA加工和影响核糖体功能。本文的在线版本（10.1186/s12870-018-1353-y）包含补充材料，可供授权用户使用。
Plastome mutants are ideal resources for elucidating the functions of plastid genes. Numerous studies have been conducted for the function of plastid genes in barley and tobacco; however, related information is limited in Chinese cabbage. A chlorophyll-deficient mutant of Chinese cabbage that was derived by ethyl methanesulfonate treatment on isolated microspores showed uniformly pale green inner leaves and slow growth compared with that shown by the wild type “Fukuda 50′ (‘FT’). Genetic analysis revealed that cdm was cytoplasmically inherited. Physiological and ultrastructural analyses of cdm showed impaired photosynthesis and abnormal chloroplast development. Utilizing next generation sequencing, the complete plastomes of cdm and ‘FT’ were respectively re-mapped to the reference genome of Chinese cabbage, and an A-to-C base substitution with a mutation ratio higher than 99% was detected. The missense mutation of plastid ribosomal protein S4 led to valine substitution for glycine at residue 193. The expression level of rps4 was analyzed using quantitative real-time PCR and found lower in than in ‘FT’. RNA gel-blot assays showed that the abundance of mature 23S rRNA, 16S rRNA, 5S rRNA, and 4.5S rRNA significantly decreased and that the processing of 23S, 16S rRNA, and 4.5S rRNA was seriously impaired, affecting the ribosomal function in cdm. These findings indicated that cdm was a plastome mutant and that chlorophyll deficiency might be due to an A-to-C base substitution of the plastome-encoded rps4 that impaired the rRNA processing and affected the ribosomal function. The online version of this article (10.1186/s12870-018-1353-y) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3.7
作者：
GAMBORG, OL;MILLER, RA;OJIMA, K
通讯作者：
OJIMA, K
DOI：
10.1016/0027-5107(90)90173-2
发表时间：
1990-07-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
BERANEK, DT
通讯作者：
BERANEK, DT
影响因子：
7.2
作者：
Azhagiri, Arun K.;Maliga, Pal
通讯作者：
Maliga, Pal
影响因子：
3.1
作者：
Colombo, Noemi;Emanuel, Carola;Boerner, Thomas
通讯作者：
Boerner, Thomas
DOI：
10.1073/pnas.2533668100
发表时间：
2003-12-23
影响因子：
11.1
作者：
Ahlert, D;Ruf, S;Bock, R
通讯作者：
Bock, R