Different from tracheophytes, liverworts commonly have mixed 35S and 5S arrays.
Different from tracheophytes, liverworts commonly have mixed 35S and 5S arrays.
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与气管植物不同，地钱通常有35S和5S混合阵列
DOI：
10.1093/aob/mcaa027
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发表时间：
2020
期刊：
影响因子：
4.2
通讯作者：
Susanne S.
中科院分区：
文献类型：
--
作者：
Aretuza;Bechteler;Temsch;Renner;Susanne S.
与真核生物中的其他核基因一样，rDNA基因（5S和35S位点）存在于每个细胞的许多拷贝中，因此，当染色时，可以提供基因组组织的基本信息。在管生植物（维管植物）中，它们通常位于单独的染色体上，即所谓的s型组织。对1791种陆地植物的分析表明，陆地植物的祖先可能是s型排列，而连锁（l型）排列可能是衍生的。然而，没有外群，只有少数蕨类和苔藓植物被包括在内。方法分析了多达12种单性或双性地茅的基因组大小和端粒、5S和35S rDNA FISH信号的分布，其中包括386个已知属中的287个。我们还使用系统发育来绘制染色体数目和明显不同性染色体的发生。本文报道了雌雄同株和雌雄同株龙葵的染色体数目。我们检测到雌雄异株plagiochila asplenioides和frulania dilatata中rDNA信号数量的性别相关差异。在后者中，雌性中存在两条UU染色体和额外的5S-35S rDNA位点导致单倍体基因组比雄性基因组大0.2082 pg；在其他雌雄异株物种中，性别特异性基因组差异很小。4种属属具有s型rDNA， 5种属属具有L-S型rDNA的混合组织，并且可能发生过多次转变，这表明rDNA位点在近缘种间并不保守。所有物种都有类似拟南芥的端粒基序，该基序的检测可以验证radula planata的染色体数目以及pinguisa和p的染色体重排。Asplenioides，后者也显示性别特异性的间质端粒重复。结论S和L rDNA排列似乎在苔类中反复进化，甚至在同一物种中也是如此。迄今为止，关于性别间rDNA积累差异的证据有限。
Background and AimsUnlike other nuclear genes in eukaryotes, rDNA genes (5S and 35S loci) are present in numerous copies per cell and, when stained, can therefore provide basic information about genome organization. In tracheophytes (vascular plants), they are usually located on separate chromosomes, the so-called S-type organization. An analysis of 1791 species of land plants suggested that S-type arrays might be ancestral in land plants, while linked (L-type) organization may be derived. However, no outgroup and only a handful of ferns and bryophytes were included.MethodsWe analysed genome sizes and the distribution of telomere, 5S and 35S rDNA FISH signals in up to 12 monoicous or dioicous species of liverworts from throughout a phylogeny that includes 287 of the 386 currently recognized genera. We also used the phylogeny to plot chromosome numbers and the occurrence of visibly distinct sex chromosomes.Key ResultsChromosome numbers are newly reported for the monoicousLejeunea cavifoliaand for females of the dioicousScapania aequiloba.We detected sex-related differences in the number of rDNA signals in the dioicousPlagiochila asplenioidesandFrullania dilatata. In the latter, the presence of two UU chromosomes in females and additional 5S-35S rDNA loci result in a haploid genome 0.2082 pg larger than the male genome; sex-specific genome differences in the other dioicous species were small. Four species have S-type rDNA, while five species have mixed L-S rDNA organization, and transitions may have occurred multiple times, as suggested by rDNA loci not being conserved among closely related species ofPellia. All species shared anArabidopsis-like telomere motif, and its detection allowed verification of the chromosome number ofRadula complanataand chromosome rearrangements inAneura pinguisandP. asplenioides, the latter also showing sex-specific interstitial telomere repeats.ConclusionsThe S and L rDNA arrangements appear to have evolved repeatedly within liverworts, even in the same species. Evidence for differential accumulation of rDNA between the sexes so far is limited.
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影响因子：
2.4
作者：
Aretuza Sousa;S. Renner
通讯作者：
Aretuza Sousa;S. Renner
DOI：
10.1002/cyto.990020311
发表时间：
1981-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
OTTO, FJ;OLDIGES, H;JAIN, VK
通讯作者：
JAIN, VK
影响因子：
3
作者：
Chacon, Juliana;Sousa, Aretuza;Renner, Susanne S.
通讯作者：
Renner, Susanne S.
影响因子：
7.2
作者：
Sousa, Aretuza;Bellot, Sidonie;Renner, Susanne S.
通讯作者：
Renner, Susanne S.
影响因子：
1.2
作者：
Aliyeva-Schnorr, Lala;Ma, Lu;Houben, Andreas
通讯作者：
Houben, Andreas