Establishment of embryonic shoot-root axis is involved in auxin and cytokinin response during Arabidopsis somatic embryogenesis.
Establishment of embryonic shoot-root axis is involved in auxin and cytokinin response during Arabidopsis somatic embryogenesis.
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DOI：
10.3389/fpls.2014.00792
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发表时间：
2014
影响因子：
5.6
通讯作者：
Zhang XS
中科院分区：
文献类型：
--
作者：
Su YH;Liu YB;Bai B;Zhang XS
生长素和细胞分裂素信号转导参与调节植物的许多发育和生理过程。植物的芽和根对这些激素有特定的反应，有时甚至是相反的反应。最近的研究已经清楚地表明，建立生长素和细胞分裂素响应信号的时空分布是控制茎尖分生组织（SAM）诱导培养组织的核心。然而，鲜为人知的是，这些激素在根尖分生组织（RAM）启动的作用。在这里，我们发现，RAM形成的几个关键调控基因的表达模式与拟南芥体细胞胚胎发生过程中胚性根分生组织的建立。有趣的是，WUS相关的HOMEOBOX 5（WOX 5）和WUSCHEL基因的早期表达被诱导，并且当体细胞胚（SE）不能被形态学鉴定时，在胚性愈伤组织中几乎重叠。它们的正确表达对于RAM和SAM的启动和胚性茎-根轴的建立是必不可少的。此外，我们分析了生长素和细胞分裂素在SE启动过程中的反应。值得注意的是，在与诱导的WOX 5表达和随后的SE形成相关的特定区域检测到细胞分裂素应答信号。拟南芥反应调节基因ARR 7和ARR 15（细胞分裂素信号传导的反馈阻遏物）的过表达，干扰RAM起始和SE诱导。这些结果为体细胞胚胎发生过程中生长素和细胞分裂素调控茎-根轴顶端-基部极性的形成提供了新的信息。
Auxin and cytokinin signaling participates in regulating a large spectrum of developmental and physiological processes in plants. The shoots and roots of plants have specific and sometimes even contrary responses to these hormones. Recent studies have clearly shown that establishing the spatiotemporal distribution of auxin and cytokinin response signals is central for the control of shoot apical meristem (SAM) induction in cultured tissues. However, little is known about the role of these hormones in root apical meristem (RAM) initiation. Here, we found that the expression patterns of several regulatory genes critical for RAM formation were correlated with the establishment of the embryonic root meristem during somatic embryogenesis in Arabidopsis. Interestingly, the early expression of the WUS-RELATED HOMEOBOX 5 (WOX5) and WUSCHEL genes was induced and was nearly overlapped within the embryonic callus when somatic embryos (SEs) could not be identified morphologically. Their correct expression was essential for RAM and SAM initiation and embryonic shoot–root axis establishment. Furthermore, we analyzed the auxin and cytokinin response during SE initiation. Notably, cytokinin response signals were detected in specific regions that were correlated with induced WOX5 expression and subsequent SE formation. Overexpression of the ARABIDOPSIS RESPONSE REGULATOR genes ARR7 and ARR15 (feedback repressors of cytokinin signaling), disturbed RAM initiation and SE induction. These results provide new information on auxin and cytokinin-regulated apical–basal polarity formation of shoot–root axis during somatic embryogenesis.
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影响因子：
9.2
作者：
Heisler, MG;Ohno, C;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
DOI：
10.1073/pnas.0811539106
发表时间：
2009-03-03
影响因子：
11.1
作者：
Pernisova, Marketa;Klima, Petr;Hejatko, Jan
通讯作者：
Hejatko, Jan
影响因子：
6.2
作者：
Bassuner, Burgund M.;Lam, Russell;Yeung, Edward C.
通讯作者：
Yeung, Edward C.
影响因子：
64.8
作者：
Hwang, I;Sheen, J
通讯作者：
Sheen, J
影响因子：
64.5
作者：
DiLaurenzio, L;WysockaDiller, J;Benfey, PN
通讯作者：
Benfey, PN