Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes
Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes
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不同形态和浓度的硒对原代培养鸡肝细胞硒酶表达和活性的调节
DOI：
10.1017/s0007114510002643
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发表时间：
2010-07
影响因子：
3.6
通讯作者：
Wei, Chengwu
中科院分区：
文献类型：
--
作者：
Pan, Cuiling;Huang, Kehe;Wu, Xianshi;Duan, Ying;Wei, Chengwu
硒酶的表达和活性受Se的调节。本研究探讨了不同形态和浓度的硒对鸡肝细胞谷胱甘肽过氧化物酶(GPx)活性及磷脂过氧化氢GPx(Gpx4)和I型脱碘酶(D1mRNA)表达的调节作用。以0(对照组)、0·5、1、1·5、2、3、4、5μ/L的0、0·5、1、1·5、2、3、4、5 Mol/Se的亚硒酸盐(Se-Met)、亚硒卡拉胶(Se-CAR)或亚硒酸钠(Na_2SeO_3)与原代培养的30~40日龄白来航鸡肝细胞单层孵育24 h。与对照组相比，Se可显著提高所有肝细胞的GPx活性，但5μ/L的Na_2SeO_3处理组肝细胞的GPx活性无明显增加，其中以2μ/μ/L的Se-Met或Se-CAR和1.5μ/L的Na_2SeO_3的作用最强。与对照组相比，Se组大鼠肝细胞Gpx4基因表达水平显著降低。与对照组相比，各剂量组的D1mRNA水平均显著升高，其中以1·5μ/L剂量组和0·5μ/L浓度组作用最强。1·5~5mmolMol/L的Se-Met对D1mRNA的影响大于同等剂量的Se-Car和μ_2SeO_3。在达到最大效应后，较高的补硒剂量可使所有经Se处理的肝细胞的GPx活性和D1mRNA水平呈剂量依赖性降低。这些结果表明，在鸡肝细胞中，不同形式和浓度的Se对GPx和D1的调节是不同的。
The expression and activity of selenoenzymes are regulated by Se. In the present study, the effects of different forms and concentrations of Se on the regulation of glutathione peroxidase (GPx) activity and phospholipid hydroperoxide GPx (GPx4) and type I deiodinase (D1) mRNA levels in chicken hepatocytes were evaluated. Primary cultured chicken hepatocyte monolayers derived from male White Leghorn chickens (aged 30–40 d) were incubated for 24 h with 0 (control), 0·5, 1, 1·5, 2, 3, 4 or 5 μmol/l of Se supplied as dl-selenomethionine (Se-Met), κ-selenocarrageenan (Se-Car) or sodium selenite (Na2SeO3). Compared with the control, Se significantly increased GPx activity in all the hepatocytes, but the activity was not increased in the hepatocytes treated with 5 μmol/l of Na2SeO3, with maximal effects being observed at 2 μmol/l of Se-Met or Se-Car and at 1·5 μmol/l of Na2SeO3, respectively. Significant decreases in GPx4 mRNA levels were observed in all the hepatocytes treated with Se (v. control). The D1 mRNA levels were significantly increased in all the groups treated with Se (v. control), with maximal effects being observed at 1·5 μmol/l of Se-Met and at 0·5 μmol/l of Se-Car or Na2SeO3, respectively. Se-Met at doses of 1·5–5 μmol/l had a greater effect on D1 mRNA than Se-Car and Na2SeO3 at equivalent doses. After resulting in a maximal effect, higher Se supplementation led to a dose-dependent reduction in GPx activity and D1 mRNA levels in all the hepatocytes treated with Se. These results suggest that in chicken hepatocytes, the regulations of GPx and D1 by different forms and concentrations of Se vary.
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影响因子：
2
作者：
Fischer, J.;Bosse, A.;Pallauf, J.
通讯作者：
Pallauf, J.
DOI：
10.1016/s1357-2725(96)00076-3
发表时间：
1996-12-01
影响因子：
4
作者：
Fujii, M;Yoshino, I;Sugano, T
通讯作者：
Sugano, T
DOI：
10.1016/j.fct.2005.10.002
发表时间：
2006-05
期刊：
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
影响因子：
--
作者：
Anna-Sigrid Keck;J. Finley
通讯作者：
Anna-Sigrid Keck;J. Finley
影响因子：
3.7
作者：
B. Kong;Hyunggee Kim;D. Foster
通讯作者：
B. Kong;Hyunggee Kim;D. Foster
影响因子：
1.5
作者：
Asheesh K. Tiwary;B. Stegelmeier;K. Panter;L. James;Jeffery O. Hall
通讯作者：
Asheesh K. Tiwary;B. Stegelmeier;K. Panter;L. James;Jeffery O. Hall