Impact of wave action and rainfall on incidence and antibiotic resistance of total coliforms in Southern California beaches.
Impact of wave action and rainfall on incidence and antibiotic resistance of total coliforms in Southern California beaches.
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DOI：
10.2166/wh.2020.100
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发表时间：
2020-10
影响因子：
2.3
通讯作者：
Dillon JG
中科院分区：
文献类型：
--
作者：
Hernandez R;Acedo I;Dillon JG
大肠菌群是娱乐沃茨中重要的细菌污染指标。对南加州海滩大肠菌群的抗生素耐药性知之甚少。本研究在受限制及非受限制波浪作用的泳滩，于干湿天气后分别从两类泳滩的岸边取样，研究泳滩的大肠菌群数目及耐腐大肠菌群的发生率。通过在mEndo琼脂上的膜过滤选择总大肠菌群，然后计数。从每个地点随机选择的菌株进行筛选，通过纸片扩散对9类抗生素的耐药性，并计算多重抗生素耐药（MAR）指数。总大肠菌群的数量显着高于下雨后相比，干燥的天气。总大肠菌群数量没有显着升高，在非限制波作用网站。与干燥天气相比，潮湿天气后，受限制的波浪作用地点的MAR指数增加了78.5%。在几乎50%的分离株中观察到对氨苄青霉素的耐药性，并且波浪作用或天气没有显着影响。最低抑菌浓度试验表明，许多菌株对氨苄青霉素高度耐药。这项研究是第一次报告在南加州海滩发现的大肠菌群的抗生素耐药性，并强调了氨苄青霉素耐药性的流行。
Coliforms are important bacterial contamination indicators in recreational waters. Little is known about the antibiotic resistance of coliforms from Southern California beaches. This study examined the numbers of coliforms as well as the incidence of antibiotic-resistant coliforms in beaches with restricted and non-restricted wave action by sampling from the shores of both types of beaches following dry and wet weather. Total coliforms were selected by membrane filtration onto mEndo agar and then enumerated. Randomly selected isolates from each location were screened for resistance to nine classes of antibiotics by disk diffusion, and the multiple antibiotic resistance (MAR) index was calculated. Numbers of total coliforms were significantly higher following rain compared to dry weather. Total coliform numbers were not significantly elevated at non-restricted wave action sites. Restricted wave action sites had a 78.5% increase in MAR index following wet weather compared to dry weather. Resistance to ampicillin was observed in almost 50% of isolates and was not significantly impacted by wave action or weather. Minimum inhibitory concentration testing revealed that many isolates were highly resistant to ampicillin. This study is the first to report on the antibiotic resistance of coliforms found in Southern California beaches and highlights the prevalence of ampicillin resistance.
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影响因子：
13.6
作者：
Hernandez, F.;Calisto-Ulloa, N.;Montory, M.
通讯作者：
Montory, M.
影响因子：
4.4
作者：
KRUMPERMAN, PH
通讯作者：
KRUMPERMAN, PH
影响因子：
5.2
作者：
Andrews, JM
通讯作者：
Andrews, JM
影响因子：
2.2
作者：
Frieswyk, Christin B.;Johnston, Carol A.;Zedler, Joy B.
通讯作者：
Zedler, Joy B.
影响因子：
5.8
作者：
Griffith, John F.;Schiff, Kenneth C.;Fuhrman, Jed A.
通讯作者：
Fuhrman, Jed A.