Estimation of chimpanzee age based on DNA methylation.
Estimation of chimpanzee age based on DNA methylation.
复制标题
DOI：
10.1038/s41598-018-28318-9
复制
发表时间：
2018-07-03
影响因子：
4.6
通讯作者：
Inoue-Murayama M
中科院分区：
文献类型：
--
作者：
Ito H;Udono T;Hirata S;Inoue-Murayama M
在野生动物保护中，了解单个动物的年龄是非常有益的。然而，对许多物种来说，估计年龄是困难的。最近，基于表观遗传学的年龄估计方法已有报道。这些研究主要针对人类，很少有关于其他动物的报道，特别是野生动物。在本研究中，一个基于ELOVL2、CCDC102B和ZNF423基因的黑猩猩年龄预测模型被开发出来，这些基因也可能在人类年龄预测中应用。用焦磷酸测序技术检测了20个黑猩猩血液样本的甲基化状态，并计算了年龄和甲基化状态之间的相关性。ELOVL2和CCDC102B基因的甲基化与年龄显著相关，并利用这些基因建立了年龄预测模型。与ELOVL2和CCDC102B组合的回归方程相比，ELOVL2回归方程的最高相关系数为0.741，平均绝对偏差为5.41，最高相关系数为0.742，MAD为5.41。虽然基于这些基因在黑猩猩身上观察到的MAD比在人类中观察到的更大，但结果表明利用DNA甲基化来估计黑猩猩的年龄是可行的，并可能对理解黑猩猩的生态和黑猩猩的保护有一定的意义。
In wild animal conservation, knowing the age of an individual animal is extremely beneficial. However, estimating the age is difficult for many species. Recently, epigenetics-based methods of estimating age have been reported. These studies were predominantly on humans with few reports on other animals, especially wild animals. In the present study, a chimpanzee (Pan troglodytes) age prediction model was developed based on the ELOVL2, CCDC102B, and ZNF423 genes that may also have application in human age prediction. Pyrosequencing was used to measure methylation in 20 chimpanzee blood samples and correlation between age and methylation status was calculated. Age and methylation of sites in ELOVL2 and CCDC102B were significantly correlated and an age prediction model was created using these genes. In the regression equation using only ELOVL2, the highest correlation coefficient was 0.741, with a mean absolute deviation (MAD) of 5.41, compared with the combination of ELOVL2 and CCDC102B, where the highest correlation coefficient was 0.742 and the MAD was 5.41. Although larger MADs were observed in chimpanzees than in humans based on these genes, the results indicate the feasibility of estimating chimpanzee age using DNA methylation, and can have implications in understanding the ecology of chimpanzees and chimpanzee conservation.
登录
查看更多内容
影响因子：
--
作者：
Xia X;Chen W;McDermott J;Han JJ
通讯作者：
Han JJ
影响因子：
7.8
作者：
Jones MJ;Goodman SJ;Kobor MS
通讯作者：
Kobor MS
DOI：
10.1098/rspb.2007.0986
发表时间：
2008-03-22
影响因子：
4.7
作者：
Nussey, Daniel H.;Wilson, Alastair J.;Kruuk, Loeske E. B.
通讯作者：
Kruuk, Loeske E. B.
影响因子：
3.7
作者：
White PA;Ikanda D;Ferrante L;Chardonnet P;Mesochina P;Cameriere R
通讯作者：
Cameriere R
影响因子：
12.3
作者：
Horvath S
通讯作者：
Horvath S