Continuous degradation of phenanthrene in cloud point system by reuse of Sphingomonas polyaromaticivorans cells
Continuous degradation of phenanthrene in cloud point system by reuse of Sphingomonas polyaromaticivorans cells
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利用鞘氨醇单胞菌细胞连续降解浊点系统中的菲
DOI：
10.1186/s13568-019-0736-2
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发表时间：
2019-01
期刊：
影响因子：
3.7
通讯作者：
Xu Meiying
中科院分区：
文献类型：
--
作者：
Pan Tao;Wang Rennv;Xiao Kun;Ye Wei;Dong Wei;Xu Meiying
多芳香鞘氨单胞菌（Sphingomonas polyaromaticivorans）对菲的萃取生物降解以前在云点系统（CPS）中进行过。在本研究中，我们探索了通过重复使用细胞和系统进行生物降解来进一步提高培养效率的可能性。采用了三种不同的回收策略。在细胞加CPS的重复使用中，细胞可重复使用3次，同时保持较高的降解率（> 90%）。此后，稀相代谢物的积累导致细胞活力下降。用新鲜培养基代替稀相，避免了这种抑制作用。然而，由于细胞对新的降解环境适应缓慢，凝聚期体积减小，细胞只能重复使用两次，活性下降。然而，同样长的降解周期（5天）的细胞再利用加上凝聚期降低了菲的整体降解效率。最后，采用3次单元加CPS重用和单个单元重用一次的组合策略，运行两个周期。3轮细胞再利用加上CPS提高了细胞利用率和菲降解效率。然后，随后的细胞重复使用可以缓解代谢物增加对细胞活力的影响。该研究为重复利用细胞连续降解CPS土壤和水中的菲提供了潜在的应用前景。
Extractive biodegradation of phenanthrene by Sphingomonas polyaromaticivorans was previously carried out in cloud point system (CPS). In this study, we explored the possibility of further increasing the efficiency of the culture by repeatedly reusing cells and the system for biodegradation. Three different recycling strategies were adopted. In reuse of cells plus CPS, cells were reused for 3 times while maintaining high degradation rates (> 90%). Thereafter, the accumulation of metabolites in the dilute phase resulted in a decrease in cell viability. This inhibition was avoided in recycling the cells plus coacervate phase by replacing the dilute phase with fresh Medium. However, due to the slow adaptation of the cells to the new degradation environment and the reduction in the volume of the coacervate phase, the cells were only reused twice and their activity decreased. However, the same long degradation cycle (5 days) as the reuse of cells plus coacervate phase reduced the overall degradation efficiency of phenanthrene. Finally, a combined strategy of 3 times of cells plus CPS reuse and individual cells reuse once was employed and run for two cycles. 3 rounds of reuse of cells plus CPS improved cells utilization and phenanthrene degradation efficiency. Then, the subsequent round of reuse of cells alone relieved the effect of increasing metabolites on cell viability. This study provides a potential application for reusing cells to continuously degrade phenanthrene in soil and water in CPS.
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浊点系统中二苯醚的萃取生物降解：污染物生物利用度增强和表面活性剂回收
影响因子：
3.2
作者：
tao pan;Chunyan liu;qiao xin;meiying xu;yangwu deng;wei dong;shuijing yu
通讯作者：
shuijing yu
影响因子：
8.7
作者：
Rabodonirina, S.;Rasolomampianina, R.;Ouddane, B.
通讯作者：
Ouddane, B.
DOI：
10.2136/sssaspecpub43.c3
发表时间：
1995
期刊：
--
影响因子：
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作者：
H. Skipper;R. Turco;Raina M. Miller
通讯作者：
H. Skipper;R. Turco;Raina M. Miller
影响因子：
4.4
作者：
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通讯作者：
J. Ortega-Calvo;Martin Alexander
影响因子：
2.9
作者：
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通讯作者：
M. G. Acedos;V. E. Santos;F. García-Ochoa