Reciprocity of Cell Mechanics with Extracellular Stimuli: Emerging Opportunities for Translational Medicine.
Reciprocity of Cell Mechanics with Extracellular Stimuli: Emerging Opportunities for Translational Medicine.
复制标题
细胞力学具有细胞外刺激的互惠性：转化医学的新兴机会。
DOI：
10.1002/smll.202107305
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发表时间：
2022-09
期刊：
影响因子：
13.3
通讯作者：
Guo, Ming
中科院分区：
文献类型：
--
作者：
Li, Yiwei;Wong, Ian Y.;Guo, Ming
关键词：
人类细胞在健康和患病组织中遇到动态力学线索，其调节其分子和生物物理表型，包括细胞内力学以及力的产生。生物/纳米材料和微流体技术的最新发展允许对细胞力学进行精密灵敏的测量，以及对外部机械刺激的严格控制以调节细胞行为。在这篇综述中，我们考虑了细胞与周围微环境双向相互作用的机械生物学，以及转化医学的潜在相关性。我们首先介绍活细胞以及细胞外基质的力学基础的关键基本概念。然后，我们专注于基于以下内容的案例研究：1）非粘附细胞变形性的微流体测量，2）微/纳米形貌上的细胞迁移，3）3D基质中细胞的牵引测量，4）类器官形态发生的机械编程，以及5）潜在治疗的主动机械刺激。这些例子突出了使用机械测量进行疾病诊断的前景，将分子与生物物理表型联系起来的系统级理解，以及基于机械扰动的治疗。最后，我们对这些新兴技术和工程、生物学和医学界面的未来方向进行了批判性的讨论。细胞力学与细胞外刺激的动态相互作用为潜在的翻译应用提供了新的机会。本文综述了机械生物学的最新进展，并强调了其应用，包括基于机械测量的疾病诊断和利用机械刺激和扰动的治疗。
Human cells encounter dynamic mechanical cues in healthy and diseased tissues, which regulate their molecular and biophysical phenotype, including intracellular mechanics as well as force generation. Recent developments in bio/nano materials and microfluidics permit exquisitely sensitive measurements of cell mechanics, as well as tight control over external mechanical stimuli to regulate cell behavior. In this review, we consider the mechanobiology of cells interacting bi-directionally with their surrounding microenvironment, and the potential relevance for translational medicine. We first introduce key fundamental concepts underlying the mechanics of living cells as well as the extracelluar matrix. We then focus on case studies based on: 1) microfluidic measurements of non-adherent cell deformability, 2) cell migration on micro/nano topographies, 3) traction measurements of cells in 3D matrix, 4) mechanical programming of organoid morphogenesis, as well as 5) active mechanical stimuli for potential therapeutics. These examples highlight the promise of disease diagnosis using mechanical measurements, a systems-level understanding linking molecular with biophysical phenotype, as well as therapies based on mechanical perturbations. We close with a critical discussion of these emerging technologies and future directions at the interface of engineering, biology, and medicine. The dynamic interplay of cell mechanics with extracellular stimuli drives new opportunities for potential translational applications. This review focuses on the most recent advances on fundamental understanding of mechanobiology, and emphasizes on its application including diagnosis of disease based on mechanical measurements and therapeutic treatments that utilize mechanical stimuli and perturbations.
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影响因子：
16.6
作者：
Chaudhuri O;Gu L;Darnell M;Klumpers D;Bencherif SA;Weaver JC;Huebsch N;Mooney DJ
通讯作者：
Mooney DJ
YAP的相位分离重新组织了基因组拓扑，以进行长期YAP靶基因表达。
影响因子：
21.3
作者：
Cai D;Feliciano D;Dong P;Flores E;Gruebele M;Porat-Shliom N;Sukenik S;Liu Z;Lippincott-Schwartz J
通讯作者：
Lippincott-Schwartz J
影响因子：
16.6
作者：
Beroz F;Jawerth LM;Münster S;Weitz DA;Broedersz CP;Wingreen NS
通讯作者：
Wingreen NS
DOI：
10.1016/j.medj.2021.08.005
发表时间：
2021-09-10
期刊：
Med (New York, N.Y.)
影响因子：
--
作者：
Bose S;Clevers H;Shen X
通讯作者：
Shen X
影响因子：
41.2
作者：
Chaudhuri O;Gu L;Klumpers D;Darnell M;Bencherif SA;Weaver JC;Huebsch N;Lee HP;Lippens E;Duda GN;Mooney DJ
通讯作者：
Mooney DJ