Transcriptome profiling of the theca interna from bovine ovarian follicles during atresia.
Transcriptome profiling of the theca interna from bovine ovarian follicles during atresia.
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DOI：
10.1371/journal.pone.0099706
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Rodgers RJ
中科院分区：
文献类型：
--
作者：
Hatzirodos N;Irving-Rodgers HF;Hummitzsch K;Rodgers RJ
内膜是一个特殊的基质层，包裹着每个生长的卵泡。它含有毛细血管、成纤维细胞、免疫细胞和类固醇生成细胞，这些细胞合成雄激素，并通过邻近的颗粒细胞转化为雌二醇。在生殖生命期间，只有少数卵泡会生长到足以排卵的大小，而大多数卵泡将经历退化/闭锁和巨噬细胞的吞噬作用。为了鉴定卵泡闭锁期间内膜中差异调节的基因，我们对来自早期窦期（<5 mm）的健康（n= 10）和窦闭锁（n =5）牛卵泡的内膜进行了转录组分析。 阵列的信号强度图的主成分分析和分级分类显示主要聚类为两组，健康组和闭锁组。在闭锁和健康内膜之间共有543个探针组差异表达。通过Intimity Pathway Analysis和Gene Ontology Enrichment Analysis Toolkit软件对这些基因的进一步分析发现，大多数表达的基因与细胞因子，激素和受体以及细胞周期和DNA复制有关。细胞周期基因编码的复制染色体复合体和有丝分裂纺锤体的组件在闭锁卵泡内膜下调，而应激反应和炎症相关的基因，如TP 53，IKBKB和TGFB 1上调。除了细胞周期调节因子外，预测被抑制的上游调节因子包括视网膜母细胞瘤1、E2转录因子1和肝细胞生长因子。我们的研究表明，在卵泡内膜中的小卵泡的窦闭锁期间，发生细胞周期和DNA复制的停滞，而不是颗粒细胞中发生的闭锁相关基因的上调。
The theca interna is a specialized stromal layer that envelops each growing ovarian follicle. It contains capillaries, fibroblasts, immune cells and the steroidogenic cells that synthesize androgens for conversion to estradiol by the neighboring granulosa cells. During reproductive life only a small number of follicles will grow to a sufficient size to ovulate, whereas the majority of follicles will undergo regression/atresia and phagocytosis by macrophages. To identify genes which are differentially regulated in the theca interna during follicular atresia, we undertook transcriptome profiling of the theca interna from healthy (n = 10) and antral atretic (n = 5) bovine follicles at early antral stages (<5 mm). Principal Component Analyses and hierarchical classification of the signal intensity plots for the arrays showed primary clustering into two groups, healthy and atretic. A total of 543 probe sets were differentially expressed between the atretic and healthy theca interna. Further analyses of these genes by Ingenuity Pathway Analysis and Gene Ontology Enrichment Analysis Toolkit software found most of the genes being expressed were related to cytokines, hormones and receptors as well as the cell cycle and DNA replication. Cell cycle genes which encode components of the replicating chromosome complex and mitotic spindle were down-regulated in atretic theca interna, whereas stress response and inflammation-related genes such as TP53, IKBKB and TGFB1 were up-regulated. In addition to cell cycle regulators, upstream regulators that were predicted to be inhibited included Retinoblastoma 1, E2 transcription factor 1, and hepatocyte growth factor. Our study suggests that during antral atresia of small follicles in the theca interna, arrest of cell cycle and DNA replication occurs rather than up- regulation of apoptosis-associated genes as occurs in granulosa cells.
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影响因子：
64.8
作者：
FALCK, B
通讯作者：
FALCK, B
影响因子：
14.9
作者：
Barrett T;Troup DB;Wilhite SE;Ledoux P;Rudnev D;Evangelista C;Kim IF;Soboleva A;Tomashevsky M;Marshall KA;Phillippy KH;Sherman PM;Muertter RN;Edgar R
通讯作者：
Edgar R
影响因子：
2.1
作者：
Irizarry, RA;Hobbs, B;Speed, TP
通讯作者：
Speed, TP
DOI：
10.1530/reprod/118.2.221
发表时间：
2000-03-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Irving-Rodgers, HF;Rodgers, RJ
通讯作者：
Rodgers, RJ
影响因子：
3.8
作者：
Irving-Rodgers, HF;Mussard, ML;Rodgers, RJ
通讯作者：
Rodgers, RJ