The Roles of Two CNG Channels in the Regulation of Ascidian Sperm Chemotaxis.
The Roles of Two CNG Channels in the Regulation of Ascidian Sperm Chemotaxis.
复制标题
两种CNG通道在海鞘精子趋化性调节中的作用
DOI：
10.3390/ijms23031648
复制
发表时间：
2022-01-31
影响因子：
5.6
通讯作者：
Inaba K
中科院分区：
文献类型：
--
作者：
Shiba K;Inaba K
精子感知并响应环境信号，以确保受精成功。信号的接收和传递迅速反映在精子鞭毛波形和游泳行为中。在海鞘Ciona Ciona（A型）中，也称为C。robusta），一种称为SAAF（精子激活和吸引因子）的卵源性硫酸化类固醇，诱导精子运动激活和趋化性。通过基因表达分析发现，Ciona testis中存在两种高表达的CNG通道：C1-tetraKCNG（四聚体，环核苷酸门控，K+选择性）和C1-HCN（超极化激活和环核苷酸门控）。为了阐明调节鞭毛运动的精子信号通路，我们重点研究了CNG通道的作用。在这项研究中，免疫化学分析表明，这两个CNG通道在玻璃海鞘精子中表达，并定位于精子鞭毛。精子运动分析和趋化过程中的Ca 2+成像表明，CNG通道抑制影响鞭毛波形和趋化转向所需的Ca 2+流出的变化。这些结果表明，玻璃海鞘精子CNG通道在调节精子运动和细胞内Ca ~（2+）的调节过程中起着至关重要的作用。
Spermatozoa sense and respond to their environmental signals to ensure fertilization success. Reception and transduction of signals are reflected rapidly in sperm flagellar waveforms and swimming behavior. In the ascidian Ciona intestinalis (type A; also called C. robusta), an egg-derived sulfated steroid called SAAF (sperm activating and attracting factor), induces both sperm motility activation and chemotaxis. Two types of CNG (cyclic nucleotide-gated) channels, Ci-tetra KCNG (tetrameric, cyclic nucleotide-gated, K+-selective) and Ci-HCN (hyperpolarization-activated and cyclic nucleotide-gated), are highly expressed in Ciona testis from the comprehensive gene expression analysis. To elucidate the sperm signaling pathway to regulate flagellar motility, we focus on the role of CNG channels. In this study, the immunochemical analysis revealed that both CNG channels are expressed in Ciona sperm and localized to sperm flagella. Sperm motility analysis and Ca2+ imaging during chemotaxis showed that CNG channel inhibition affected the changes in flagellar waveforms and Ca2+ efflux needed for the chemotactic turn. These results suggest that CNG channels in Ciona sperm play a vital role in regulating sperm motility and intracellular Ca2+ regulation during chemotaxis.
登录
查看更多内容
影响因子：
0.7
作者：
Darszon, Alberto;Guerrero, Adan;Wood, Christopher D.
通讯作者：
Wood, Christopher D.
影响因子：
--
作者：
Hozumi, Akiko;Padma, Potturi;Inaba, Kazuo
通讯作者：
Inaba, Kazuo
影响因子：
2.1
作者：
Oishi, T;Tsuchikawa, H;Morisawa, M
通讯作者：
Morisawa, M
影响因子：
5.6
作者：
Shiba K;Inaba K
通讯作者：
Inaba K
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro