The effect of AMP kinase activation on differentiation and maturation of osteoblast cultured on titanium plate.
The effect of AMP kinase activation on differentiation and maturation of osteoblast cultured on titanium plate.
复制标题
AMP激酶激活对钛板上成骨细胞分化成熟的影响。
DOI：
10.1016/j.jds.2021.12.003
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发表时间：
2022-07
影响因子：
3.5
通讯作者：
Kido, Hirofumi
中科院分区：
文献类型：
--
作者：
Vansana, Phanthavong;Kakura, Kae;Taniguchi, Yusuke;Egashira, Kei;Matsuzaki, Etsuko;Tsutsumi, Takashi;Kido, Hirofumi
关键词：
5‘-腺苷一磷酸活化蛋白激酶(AMPK)是一种维持细胞内稳态的酶，具有多种生物学活性。本研究的目的是评价AMPK激活对种植体预后的影响。用24孔板在钛表面培养MC3T3-E1成骨样细胞。实验分为3组：(1)正常培养组(对照组)，(2)成骨诱导组，(3)成骨诱导+AMPK激活组。分别于实验后第0、7、14、21天测定细胞计数，实时定量聚合酶链式反应检测成骨相关基因ALP和Osterix的表达。此外，还评估了ALP活性和钙化情况。实时荧光定量聚合酶链式反应检测结果显示，与正常培养或成骨诱导相比，AMPK激活后，作为钙化初期标志的碱性磷酸酶的表达显著增加。Osterix的表达也显著增加，这是钙化后期的一个标志。由于观察到ALP活性和钙化潜能显著增加，这表明AMPK激活可以诱导成骨细胞钙化潜能的增加。AMPK激活促进种植体外周成骨细胞分化成熟，促进钙化。我们的结果表明，AMPK的激活可能有助于维持种植体的稳定性。
5′ Adenosine monophosphate-activated protein kinase (AMPK) is known as an enzyme that maintains intracellular homeostasis and has various biological activity. The purpose of this study is evaluation effect of AMPK activation on implant prognosis. MC3T3-E1 osteoblast-like cells were cultured on titanium using a 24-well plate. The experimental group was divided into the following 3 groups: (1) the normal culture group (control group), (2) the osteogenic induction group, and (3) the osteogenic induction + AMPK activation group. The cell counts were measured; real-time PCR was used to assess the expression of ALP and Osterix as osteogenic related genes at Day 0,7,14 and 21 after experiments. Additionally, ALP activity and calcification were assessed. The results of the real-time PCR assessments revealed that the expression of ALP, which is a marker for the initial stages of calcification, was significantly increased by AMPK activation compared to the normal culture or osteogenic induction. A significant increase was also observed in the expression of Osterix, which is a marker for the later stages of calcification. Because significant increases were observed in ALP activity and calcification potential, this suggested that AMPK activation could elicit an increase in osteoblast calcification potential. AMPK activation promotes implant peripheral osteoblast differentiation and maturation and enhances calcification. Our results suggest that AMPK activation may help to maintain implant stability.
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影响因子：
7.5
作者：
Zhou X;Xu SN;Yuan ST;Lei X;Sun X;Xing L;Li HJ;He CX;Qin W;Zhao D;Li PQ;Moharomd E;Xu X;Cao HL
通讯作者：
Cao HL
影响因子：
5.7
作者：
Cervero, Claudia;Montull, Neus;Caldero, Jordi
通讯作者：
Caldero, Jordi
DOI：
10.1016/j.bbrc.2010.12.099
发表时间：
2011-01-28
影响因子：
3.1
作者：
Jang, Won Gu;Kim, Eun Jung;Koh, Jeong-Tae
通讯作者：
Koh, Jeong-Tae
多层 BMP-2 基因涂层多孔钛种植体表面的骨反应
影响因子：
4.3
作者：
Jiang, Qiao-Hong;Liu, Li;He, Fu-Ming
通讯作者：
He, Fu-Ming
影响因子：
1.6
作者：
Ehrenfest, David M. Dohan;Vazquez, Lydia;Bernard, Jean-Pierre
通讯作者：
Bernard, Jean-Pierre