Isolation and purification of aloe anthraquinones based on an ionic liquid/salt aqueous two-phase system
Isolation and purification of aloe anthraquinones based on an ionic liquid/salt aqueous two-phase system
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基于离子液体/盐水两相体系分离纯化芦荟蒽醌类化合物
DOI：
10.1016/j.seppur.2012.06.021
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发表时间：
2012-09
影响因子：
8.6
通讯作者：
Xuelei Xu
中科院分区：
文献类型：
--
作者：
Zhijian Tan;Fenfang Li;Xuelei Xu
探索温和、高效的天然植物活性成分分离纯化技术仍在进行中。离子液体是一种新型的室温下呈液态的熔盐，具有许多独特的性质，是一种安全、环保的化学品。采用离子液体和无机盐组成的双水相体系分离纯化芦荟叶中的蒽醌类化合物。通过对萃取过程的研究，考察了影响萃取效果的主要因素：盐的种类和质量、体系的pH值、萃取温度和平衡时间。芦荟AQs优先迁移到IL-丰富的阶段。在最佳条件下，[C4 mim] BF 4/Na 2SO 4体系在25°C、pH 4.0时对总蒽醌的最大萃取率为92.1%。通过调节pH值，回收IL进行反萃取实验。并采用高效液相色谱法对芦荟大黄素和大黄酚的主要成分进行了分析。离子液体基ATPSs（ILATPSs）是典型的液-液萃取中替代挥发性有机化合物的很好的候选者。该提取技术为天然植物或生物样品中其他活性成分的纯化开辟了新的可能性。
To explore mild and efficient techniques for separation and purification of the active ingredients in natural plants is still ongoing. Ionic liquid (IL) is a new class of molten salts that are liquid at ambient temperature, which has many unique properties is safe and environmentally friendly chemical. Aqueous two-phase systems (ATPSs) based on ILs and salts were applied to isolate and purify anthraquinones (AQs) from aloe leaves. The main affecting parameters on the partitioning behavior and extraction efficiency were investigated through the partitioning process: type and mass of salt, pH, extraction temperature, and equilibrium time of the systems. Aloe AQs preferentially migrated into the IL-rich phase. Under the optimal conditions, the maximal extraction efficiency 92.1% of total AQs was obtained with the [C4mim]BF4/Na2SO4system at 25°C and pH 4.0. The reverse extraction experiments were done by adjusting pH with IL being recycled. Furthermore, the major constituents of aloe-emodin and chrysophanol were analyzed by a HPLC method. Ionic liquid-based ATPSs (ILATPSs) are great candidates for the replacement of volatile organic compounds in typical liquid–liquid extraction. This proposed extraction technique opens up new possibilities in purification of other active ingredients in natural plants or biologic samples.
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