Transcriptomic response to ISAV infection in the gills, head kidney and spleen of resistant and susceptible Atlantic salmon.
Transcriptomic response to ISAV infection in the gills, head kidney and spleen of resistant and susceptible Atlantic salmon.
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DOI：
10.1186/s12864-022-09007-4
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发表时间：
2022-11-28
期刊：
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
传染性鲑鱼贫血病毒（ISAV）是一种导致大西洋鲑鱼（萨尔莫salar）水产养殖大量损失的正粘病毒。目前可用的治疗方法和疫苗并不完全有效，因此选择性育种以产生抗ISAV的大西洋鲑鱼品系是该行业的高度优先事项。基因组选择和潜在的基因组编辑可以应用于提高水产养殖种群的抗病性，这两种方法都可以受益于对ISAV抗性基因组机制的更多了解。为了提高我们对大西洋鲑鱼抗ISAV的机制的理解，我们在ISAV感染的鲑鱼中进行了转录组学研究，对比了对这种病毒的抗性水平。在三个时间点（攻击前、攻击后7天和14天）收集了12条抗性鱼和12条易感鱼的三种不同组织（鳃、头肾和脾脏）并进行RNA测序。在每个时间点比较感染和未感染的鱼以及抗性和易感鱼的转录组。结果表明，对ISAV的反应是器官特异性的;在头肾中观察到对感染的重要反应，免疫过程如干扰素和NLR途径上调，而在鳃和脾脏中，反应更为温和。除了免疫相关基因，我们的研究结果表明，其他过程，如泛素化和核糖体加工是重要的，在早期感染ISAV。此外，抗病和易感鱼类之间的比较也突出了一些有趣的基因相关的泛素化，细胞内运输和炎性小体。大西洋鲑鱼感染ISAV揭示了器官特异性反应，这意味着在感染过程中的差异功能。在这些早期时间点，在头肾中观察到免疫应答，而相比之下，鳃和脾脏显示出适度的应答。耐药和敏感样本之间的比较突出了进一步研究的感兴趣的基因，例如与泛素化或炎性体相关的基因。在线版本包含补充材料，可通过10.1186/s12864-022-09007-4获得。
Infectious Salmon Anaemia virus (ISAV) is an orthomyxovirus responsible for large losses in Atlantic salmon (Salmo salar) aquaculture. Current available treatments and vaccines are not fully effective, and therefore selective breeding to produce ISAV-resistant strains of Atlantic salmon is a high priority for the industry. Genomic selection and potentially genome editing can be applied to enhance the disease resistance of aquaculture stocks, and both approaches can benefit from increased knowledge on the genomic mechanisms of resistance to ISAV. To improve our understanding of the mechanisms underlying resistance to ISAV in Atlantic salmon we performed a transcriptomic study in ISAV-infected salmon with contrasting levels of resistance to this virus. Three different tissues (gills, head kidney and spleen) were collected on 12 resistant and 12 susceptible fish at three timepoints (pre-challenge, 7 and 14 days post challenge) and RNA sequenced. The transcriptomes of infected and non-infected fish and of resistant and susceptible fish were compared at each timepoint. The results show that the responses to ISAV are organ-specific; an important response to the infection was observed in the head kidney, with up-regulation of immune processes such as interferon and NLR pathways, while in gills and spleen the response was more moderate. In addition to immune related genes, our results suggest that other processes such as ubiquitination and ribosomal processing are important during early infection with ISAV. Moreover, the comparison between resistant and susceptible fish has also highlighted some interesting genes related to ubiquitination, intracellular transport and the inflammasome. Atlantic salmon infection by ISAV revealed an organ-specific response, implying differential function during the infection. An immune response was observed in the head kidney in these early timepoints, while gills and spleen showed modest responses in comparison. Comparison between resistance and susceptible samples have highlighted genes of interest for further studies, for instance those related to ubiquitination or the inflammasome. The online version contains supplementary material available at 10.1186/s12864-022-09007-4.
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影响因子：
4.4
作者：
Gervais O;Barria A;Papadopoulou A;Gratacap RL;Hillestad B;Tinch AE;Martin SAM;Robledo D;Houston RD
通讯作者：
Houston RD
影响因子：
6.7
作者：
Cabral JM;Cushman CH;Sodroski CN;Knipe DM
通讯作者：
Knipe DM
影响因子：
4.4
作者：
Aamelfot M;McBeath A;Christiansen DH;Matejusova I;Falk K
通讯作者：
Falk K
影响因子：
46.9
作者：
Bray, Nicolas L.;Pimentel, Harold;Pachter, Lior
通讯作者：
Pachter, Lior
影响因子：
1.4
作者：
Bouchard, D;Keleher, W;Nicholson, BL
通讯作者：
Nicholson, BL