The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study
The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study
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使用非靶向和广泛靶向代谢组学来区分大鼠死因窒息和心源性猝死：初步研究
DOI：
10.1016/j.arabjc.2022.104322
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发表时间：
2022-10
影响因子：
6
通讯作者：
Huang Pin
中科院分区：
文献类型：
--
作者：
Zhang Kai;Tuo Ya;Liu Ruina;Yan Hui Huang Pin;Xiang Ping;Wang Zhenyuan;Huang Pin
在窒息的情况下，确定死亡原因很重要。然而，很难得出窒息死亡的结论，特别是当死者有潜在的心脏病时，因为窒息和心源性猝死（SCD）通常没有特异性和代表性的尸体体征。本工作的目的是调查代谢组学的潜力，以区分窒息SCD的死亡原因。共30只雄性Sprague-Dawley大鼠用于构建窒息、SCD（干扰性死亡原因）和颈椎脱位（对照）模型。本研究采用非靶向和广泛靶向代谢组学方法获得大鼠肺代谢谱。首先，研究窒息引起的代谢改变。在碳水化合物代谢、内分泌系统和感觉系统中发现了显著的变化。其次，我们筛选潜在的生物标志物并建立分类模型来确定死亡原因。此外，一些生物标志物在死后24 h和48 h仍然存在差异，因此死亡后仍可确定死因。这项研究显示了代谢组学在研究死亡过程中发生的代谢变化以及在死亡后根据代谢差异确定死亡原因方面的应用潜力。
It is important to determine the cause of death in the case of asphyxia. However, it is difficult to conclude death by asphyxia, especially when the deceased has underlying heart disease, because there are often no specific and representative corpse signs for both asphyxia and sudden cardiac death (SCD). The aim of the present work was to investigate the potential of metabolomics to discriminate asphyxia from SCD as the cause of death. A total of thirty male Sprague–Dawley rats were used to construct models of asphyxia, SCD (interfering cause of death), and cervical dislocation (control). Untargeted and widely targeted metabolomics approaches were used to obtain rat pulmonary metabolic profiles in this study. First, the metabolic alterations resulting from asphyxia were explored. There were significant changes found in carbohydrate metabolism, the endocrine system, and the sensory system. Second, we screened potential biomarkers and built classification models to determine the cause of death. Moreover, some biomarkers remained differentiated at 24 h and 48 h postmortem, so the cause of death could still be determined after death. This study showed the application potential of metabolomics to investigate the metabolic changes occurring in the process of death, as well as to determine the cause of death on the basis of metabolic differences even after death.
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影响因子：
24
作者：
Kong, Melissa H.;Fonarow, Gregg C.;Peterson, Eric D.;Curtis, Anne B.;Hernandez, Adrian F.;Sanders, Gillian D.;Thomas, Kevin L.;Hayes, David L.;Al-Khatib, Sana M.
通讯作者：
Al-Khatib, Sana M.
影响因子：
2.1
作者：
Chighine A;Porcu M;Ferino G;Lenigno N;Trignano C;d'Aloja E;Locci E
通讯作者：
Locci E
心脏组织中 miRNA-3185/CYP4A11 轴的鉴定作为机械性窒息的生物标志物
影响因子：
2.2
作者：
Han, Liujun;Zhang, Heng;Chen, Long
通讯作者：
Chen, Long
影响因子：
2.2
作者：
Qi Wang;T. Ishikawa;T. Michiue;B. Zhu;D. Guan;H. Maeda
通讯作者：
Qi Wang;T. Ishikawa;T. Michiue;B. Zhu;D. Guan;H. Maeda
DOI：
10.1016/b978-0-12-814208-0.00005-1
发表时间：
2022
期刊：
Mechanisms of Cell Death and Opportunities for Therapeutic Development
影响因子：
--
作者：
Daiqing Liao
通讯作者：
Daiqing Liao