Lingual and palatal gustatory afferents each depend on both BDNF and NT-4, but the dependence is greater for lingual than palatal afferents.
Lingual and palatal gustatory afferents each depend on both BDNF and NT-4, but the dependence is greater for lingual than palatal afferents.
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DOI：
10.1002/cne.22400
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发表时间：
2010-08-15
期刊：
影响因子：
--
通讯作者：
Krimm RF
中科院分区：
文献类型：
--
作者：
Patel AV;Huang T;Krimm RF
膝状体神经节的神经元支配位于两个空间上不同的目标，舌头和腭味蕾。在Bdnf−/−小鼠和Ntf 4/5−/−小鼠中，大约50%的神经元死亡。Bdnf−/−/Ntf 4/5−/−双突变体失去90-95%的膝状体神经节神经元。为了确定不同的亚群是否受到神经营养因子的不同影响，我们分别定量了两个神经节亚群的神经元，并在野生型，Bdnf−/−，Ntf 4/5−/−和Bdnf−/−/Ntf 4/5−/−小鼠的每个目标区域内出生时剩余的味蕾。在野生型小鼠中，相同数量的神经元支配着前舌和软腭，每个目标包含相同数量的味蕾。与野生型小鼠相比，Bdnf−/−小鼠显示出支配舌头的膝状体神经元减少了50%，支配软腭的神经元减少了28%。Ntf 4/5−/−小鼠失去了58%的支配舌头的神经元和41%的支配软腭的神经元。与BDNF缺失小鼠相比，NT-4缺失小鼠的味蕾丧失并不严重。Bdnf−/−/Ntf 4/5−/−小鼠的舌头由0至4个味觉神经元支配，出生时含有3至16个味蕾，这表明即使所有神经支配都失去了，一些味蕾仍然存在。因此，味觉神经元同样依赖于BDNF和NT-4表达的生存，无论他们支配的外周靶点。然而，味蕾对BDNF比NT-4去除更敏感。
Neurons of the geniculate ganglion innervate taste buds located in two spatially distinct targets, the tongue and palate. About 50% of these neurons die in Bdnf−/− mice and Ntf4/5−/− mice. Bdnf−/−/Ntf4/5−/− double mutants lose 90-95% of geniculate ganglion neurons. To determine whether different subpopulations are differentially influenced by neurotrophins, we quantified neurons from two ganglion subpopulations separately and remaining taste buds at birth within each target field in wild-type, Bdnf−/−, Ntf4/5−/−, and Bdnf−/−/Ntf4/5−/− mice. In wild-type mice the same number of neurons innervated the anterior tongue and soft palate and each target contained the same number of taste buds. Compared to wild-type mice, Bdnf−/− mice showed a 50% reduction in geniculate neurons innervating the tongue and a 28% loss in neurons innervating the soft palate. Ntf4/5−/− mice lost 58% of the neurons innervating the tongue and 41% of the neurons innervating the soft palate. Taste bud loss was not as profound in the NT-4 null mice compared to BDNF-null mice. Tongues of Bdnf−/−/Ntf4/5−/− mice were innervated by 0 to 4 gustatory neurons and contained 3 to 16 taste buds at birth, indicating that some taste buds remain even when all innervation is lost. Thus, gustatory neurons are equally dependent on BDNF and NT-4 expression for survival, regardless of what peripheral target they innervate. However, taste buds are more sensitive to BDNF than NT-4 removal.
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DOI：
10.1073/pnas.77.7.4113
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRULET, P;BABINET, C;JACOB, F
通讯作者：
JACOB, F
影响因子：
2.7
作者：
Krimm, RF;Miller, KK;Albers, KM
通讯作者：
Albers, KM
DOI：
10.1073/pnas.79.7.2328
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRULET, P;JACOB, F
通讯作者：
JACOB, F
影响因子：
2.5
作者：
Ito, Akira;Nosrat, Irina V.;Nosrat, Christopher A.
通讯作者：
Nosrat, Christopher A.
影响因子：
2.9
作者：
MAGIN, TM;JORCANO, JL;FRANKE, WW
通讯作者：
FRANKE, WW