Magnetic resonance imaging of soft tissue infection with iron oxide labeled granulocytes in a rat model.
Magnetic resonance imaging of soft tissue infection with iron oxide labeled granulocytes in a rat model.
复制标题
用氧化铁标记为大鼠模型中的粒细胞的软组织感染的磁共振成像。
DOI：
10.1371/journal.pone.0051770
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Haverich A
中科院分区：
文献类型：
--
作者：
Baraki H;Zinne N;Wedekind D;Meier M;Bleich A;Glage S;Hedrich HJ;Kutschka I;Haverich A
我们试图使用粒细胞通过磁共振成像（MRI）来检测大鼠的急性软组织感染，粒细胞之前用氧化铁超顺磁性颗粒（SPIO）标记。通过皮下接种金黄色葡萄球菌悬浮液诱导大鼠胸骨旁感染。从同基因供体大鼠中分离的粒细胞用 SPIO 标记。在静脉注射 SPIO 标记或未标记的粒细胞之前、6 小时和 12 小时后，对感染的大鼠进行 MRI 成像。 MR 结果与普鲁士蓝染色的组织学分析以及通过磁性细胞分离从感染区域重新分离 SPIO 标记的粒细胞相关。实验组所有动物的对比后 T2* 加权 MR 图像上的感染部位均存在敏感性效应。粒细胞给药后 6 小时检测到 MRI 信号强度 (SI) 降低的区域，并在 12 小时时更为明显。通过感染组织中的普鲁士蓝染色鉴定出 SPIO 标记的粒细胞，并且可以通过磁性细胞分离成功地从感染区域中重新分离出 SPIO 标记的粒细胞。 SPIO 标记的粒细胞在 MRI 中的应用为检测早期感染过程的诊断特异性和敏感性提供了新的视角。
We sought to detect an acute soft tissue infection in rats by magnetic resonance imaging (MRI) using granulocytes, previously labeled with superparamagnetic particles of iron oxide (SPIO). Parasternal infection was induced by subcutaneous inoculation of Staphylococcus aureus suspension in rats. Granulocytes isolated from isogenic donor rats were labeled with SPIO. Infected rats were imaged by MRI before, 6 and 12 hours after intravenous injection of SPIO-labeled or unlabeled granulocytes. MR findings were correlated with histological analysis by Prussian blue staining and with re-isolated SPIO-labeled granulocytes from the infectious area by magnetic cell separation. Susceptibility effects were present in infected sites on post-contrast T2*-weighted MR images in all animals of the experimental group. Regions of decreased signal intensity (SI) in MRI were detected at 6 hours after granulocyte administration and were more pronounced at 12 hours. SPIO-labeled granulocytes were identified by Prussian blue staining in the infected tissue and could be successfully re-isolated from the infected area by magnetic cell separation. The application of SPIO-labeled granulocytes in MRI offers new perspectives in diagnostic specificity and sensitifity to detect early infectious processes.
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影响因子：
3.3
作者：
Bowen, CV;Zhang, XW;Rutt, BK
通讯作者：
Rutt, BK
DOI：
10.1102/1470-7330.2008.9018
发表时间：
2008-10-04
期刊：
Cancer imaging : the official publication of the International Cancer Imaging Society
影响因子：
--
作者：
Heiken JP
通讯作者：
Heiken JP
影响因子：
2.1
作者：
Bierry, Guillaume;Huang, Ambrose J.;Bredella, Miriam A.
通讯作者：
Bredella, Miriam A.
影响因子：
19.7
作者：
Bierry, Guillaume;Jehl, Francois;Kremer, Stephane
通讯作者：
Kremer, Stephane
DOI：
10.1016/j.ejvs.2010.05.016
发表时间：
2010-09-01
影响因子：
5.7
作者：
Bruggink, J. L. M.;Glaudemans, A. W. J. M.;Zeebregts, C. J.
通讯作者：
Zeebregts, C. J.