Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma.
Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma.
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DOI：
10.1007/s00216-010-3979-y
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发表时间：
2010-09
影响因子：
4.3
通讯作者：
Muddiman, David C.
中科院分区：
文献类型：
--
作者：
Hawkridge, Adam M.;Wysocky, Rebecca B.;Petitte, James N.;Anderson, Kenneth E.;Mozdziak, Paul E.;Fletcher, Oscar J.;Horowitz, Jonathan M.;Muddiman, David C.
关键词：
家鸡（Gallus arcticus）已成为研究自发性上皮性卵巢癌（EOC）的发病和进展的有力实验模型，其疾病患病率在2-7岁之间可超过35%。本文报道了一种用于生物标志物发现的新实验策略，其结合了EOC的鸡模型、具有匹配组织的纵向血浆样品收集、基于先进质谱的蛋白质组学以及源自个体性指数的概念。从2.5岁开始，每三个月从148只年龄匹配的鸡中抽取血液，持续一年。在一年样本采集期结束时，对73只存活的鸡实施安乐死，进行尸检，并采集组织。对这73只鸡的切除组织进行病理学评估，证实5只鸡（6.8%）发生了EOC。使用蛋白质组学工作流程（包括凝胶内消化、nanoLC与高性能质谱联用以及无标记（光谱计数））来测量鸡血浆蛋白质组的生物个体内变异性（CVW）。本研究选择了来自73只鸟生物储存库内两只鸟的纵向血浆样品组;一只鸟被认为是“健康的”，第二只鸟发展为晚期EOC。共鉴定了来自未耗尽血浆的116种蛋白质，其中80种蛋白质在所有样品组中共有。使用多次分析的单个血浆样品测量无标记蛋白质组学工作流程的运行内和运行间分析变异性（CVA）。发现卵巨球蛋白（ovostatin）在晚期EOC鸟中增加超过6个月，为进一步研究提供了初始候选蛋白。
The domestic chicken (Gallus domesticus) has emerged as a powerful experimental model for studying the onset and progression of spontaneous epithelial ovarian cancer (EOC) with a disease prevalence that can exceed 35% between 2-7 years of age. A novel experimental strategy for biomarker discovery is reported herein that combines the chicken model of EOC, longitudinal plasma sample collection with matched tissues, advanced mass spectrometry-based proteomics, and concepts derived from the index of individuality. Blood was drawn from 148 age-matched chickens starting at 2.5 years of age every three months for one-year. At the conclusion of the one-year sample collection period, the 73 birds that remained alive were euthanized, necropsied, and tissues were collected. Pathological assessment of resected tissues from these 73 birds confirmed that 5 birds (6.8%) developed EOC. A proteomics workflow including in-gel digestion, nanoLC coupled to high performance mass spectrometry, and label-free (spectral counting) was used to measure the biological intra-individual variability (CVW) of the chicken plasma proteome. Longitudinal plasma sample sets from two birds within the 73-bird biorepository were selected for this study; one bird was considered “healthy” and the second bird developed late-stage EOC. A total of 116 proteins from un-depleted plasma were identified with 80 proteins shared among all sample sets. Within- and between-run analytical variability (CVA) of the label-free proteomics workflow was measured using a single plasma sample analyzed multiple times. Ovomacroglobulin (ovostatin) was found to increase over a 6 month period in the late-stage EOC bird providing an initial candidate protein for further investigation.
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DOI：
10.1038/nrc2644
发表时间：
2009-06
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.2
作者：
Andersen MR;Goff BA;Lowe KA;Scholler N;Bergan L;Dresher CW;Paley P;Urban N
通讯作者：
Urban N
影响因子：
11.2
作者：
Faça VM;Hanash SM
通讯作者：
Hanash SM
DOI：
10.1016/j.jchromb.2009.02.040
发表时间：
2009-04-01
影响因子：
3
作者：
Andrews, Genna L.;Shuford, Christopher M.;Burnett, John C., Jr.;Hawkridge, Adam M.;Muddiman, David C.
通讯作者：
Muddiman, David C.
影响因子：
82.9
作者：
Dinulescu, DM;Ince, TA;Jacks, T
通讯作者：
Jacks, T