Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus.
Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus.
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DOI：
10.1673/031.012.9101
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Liu ZX
中科院分区：
文献类型：
--
作者：
Zhang YL;Zhang SZ;Kulye M;Wu SR;Yu NT;Wang JH;Zeng HM;Liu ZX
RNA干扰（RNAi）是一种进行功能基因组研究的技术，也是一种潜在的作物保护工具。发展可靠的生产和递送双链RNA（dsRNA）的方法是有效防治害虫的主要挑战。在这项研究中，用在细菌中表达或体外合成的CiHR 3 dsRNA喂养二化螟（Crambidae：鳞翅目）。C. infuscatellus成功触发了昆虫生长发育的重要基因--蜕皮调节转录因子CiHR 3的沉默，并在处理7天内导致昆虫出现明显异常和体重减轻。该研究是利用细菌表达的dsRNA或体外合成的dsRNA介导的基于摄食的RNAi的理想实例。结果还表明，基于摄食的RNA干扰是一种潜在的控制C。infuscatellus。
RNA interference (RNAi) is a technology for conducting functional genomic studies and a potential tool for crop protection against insect pests. Development of reliable methods for production and delivery of double-stranded RNA (dsRNA) is the major challenge for efficient pest control. In this study, Chilo infuscatellus Snellen (Crambidae: Lepidoptera) was fed with CiHR3 dsRNA expressed in bacteria or synthesized in vitro. The dsRNA ingested by C. infuscatellus successfully triggered silencing of the molt-regulating transcription factor CiHR3, an important gene for insect growth and development, and caused significant abnormalities and weight loss in insects within seven days of treatment. This study is an ideal example of feeding-based RNAi mediated by dsRNA expressed in bacteria or synthesized in vitro. The results also suggested that feeding-based RNA interference is a potential method for the management of C. infuscatellus.
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影响因子：
3.8
作者：
Krishnan, Chandraraj;Sousa, Leonardo da Costa;Balan, Venkatesh
通讯作者：
Balan, Venkatesh
影响因子：
--
作者：
Sullivan, AA;Thummel, CS
通讯作者：
Thummel, CS
影响因子：
3.7
作者：
Tian H;Peng H;Yao Q;Chen H;Xie Q;Tang B;Zhang W
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Zhang W
影响因子：
14.9
作者：
Marchler-Bauer A;Lu S;Anderson JB;Chitsaz F;Derbyshire MK;DeWeese-Scott C;Fong JH;Geer LY;Geer RC;Gonzales NR;Gwadz M;Hurwitz DI;Jackson JD;Ke Z;Lanczycki CJ;Lu F;Marchler GH;Mullokandov M;Omelchenko MV;Robertson CL;Song JS;Thanki N;Yamashita RA;Zhang D;Zhang N;Zheng C;Bryant SH
通讯作者：
Bryant SH
影响因子：
6.2
作者：
Arvinth, S.;Arun, S.;Subramonian, N.
通讯作者：
Subramonian, N.