Molecular and cellular mechanisms involved in the Trypanosoma cruzi/host cell interplay.
Molecular and cellular mechanisms involved in the Trypanosoma cruzi/host cell interplay.
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DOI：
10.1002/iub.1019
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发表时间：
2012-05
期刊：
影响因子：
4.6
通讯作者：
Isabel Colombo, Maria
中科院分区：
文献类型：
--
作者：
Silvia Romano, Patricia;Agustin Cueto, Juan;Florencia Casassa, Ana;Cristina Vanrell, Maria;Gottlieb, Roberta A.;Isabel Colombo, Maria
原生动物寄生虫克氏锥虫有一个复杂的生物循环，涉及脊椎动物和无脊椎动物宿主。在哺乳动物中，这种寄生虫的感染性类鞭毛虫形式可以通过利用吞噬样或非吞噬机制入侵几种细胞类型，具体取决于所涉及的细胞类别。形态研究表明，当锥体鞭毛虫接触巨噬细胞时，它们诱导形成不同于非感染性上鞭毛虫的典型吞噬作用的质膜突起。相反，当类鞭毛虫感染上皮细胞或肌肉细胞时，细胞表面的修饰最小，这表明诱导了不同类别的过程。溶酶体依赖或独立的克氏毛滴虫侵袭宿主细胞是两种不同的模型，描述了寄生虫进入非吞噬细胞时激活的分子和细胞事件。在此背景下，我们先前已经表明，在感染前在宿主细胞中诱导自噬有利于克氏毛滴虫的入侵。此外，我们还证明了自噬小体和自噬蛋白Lc3被募集到克氏锥虫的进入部位，并且新形成的克氏锥虫寄生液泡具有自噬溶酶体的特征。本文就克氏毛滴虫侵袭非吞噬细胞的分子和细胞机制的研究进展作一综述。基于我们的发现，我们提出了一个新的模型，在该模型中，克鲁兹毛虫利用饥饿期间自噬的上调来增加其对宿主细胞的成功定植。
The protozoan parasite Trypanosoma cruzi has a complex bi-ological cycle that involves vertebrate and invertebrate hosts. In mammals, the infective trypomastigote form of this parasite can invade several cell types by exploiting phagocytic-like or non-phagocytic mechanisms depending on the class of cell involved. Morphological studies showed that when trypomastigotes contact macrophages, they induce the formation of plasma membrane protrusions that differ from the canonical phagocytosis that occurs in the case of noninfective epimastigotes. In contrast, when trypomastigotes infect epithelial or muscle cells, the cell surface is minimally modified, suggesting the induction of a different class of process. Lysosomal-dependent or -independent T. cruzi invasion of host cells are two different models that describe the molecular and cellular events activated during parasite entry into nonphagocytic cells. In this context, we have previously shown that induction of autophagy in host cells before infection favors T. cruzi invasion. Furthermore, we demonstrate that autophagosomes and the autophagosomal protein LC3 are recruited to the T. cruzi entry sites and that the newly formed T. cruzi parasitophorous vacuole has characteristics of an autophagolysosome. This review summarizes the current knowledge of the molecular and cellular mechanisms of T. cruzi invasion in nonphagocytic cells. Based on our findings, we propose a new model in which T. cruzi takes advantage of the up-regulation of autophagy during starvation to increase its successful colonization of host cells.
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Dynasore是一种动力蛋白抑制剂，可抑制克鲁齐锥虫进入腹膜巨噬细胞。
影响因子：
3.7
作者：
Barrias ES;Reignault LC;De Souza W;Carvalho TM
通讯作者：
Carvalho TM
影响因子：
2.4
作者：
MILDER, R;KLOETZEL, J
通讯作者：
KLOETZEL, J
影响因子：
3.1
作者：
Coimbra, Vanessa C.;Yamamoto, Denise;Rabinovitch, Michel
通讯作者：
Rabinovitch, Michel
DOI：
10.1111/j.1550-7408.1976.tb03835.x
发表时间：
1976-01-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
DVORAK, JA;HOWE, CL
通讯作者：
HOWE, CL
影响因子：
3.3
作者：
Mizushima, N;Yamamoto, A;Ohsumi, Y
通讯作者：
Ohsumi, Y