Genome-wide comparative analysis of papain-like cysteine protease family genes in castor bean and physic nut.
Genome-wide comparative analysis of papain-like cysteine protease family genes in castor bean and physic nut.
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蓖麻子和物理坚果中木瓜蛋白酶样半胱氨酸蛋白酶家族基因的全基因组比较分析
DOI：
10.1038/s41598-017-18760-6
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发表时间：
2018-01-10
影响因子：
4.6
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Zou Z;Huang Q;Xie G;Yang L
木瓜蛋白酶样半胱氨酸蛋白酶(PLCP)是一类参与多种植物过程的蛋白水解酶。与拟南芥的广泛研究相比，在蓖麻和麻疯树这两种大豆科植物中鲜为人知，几乎没有最近的全基因组复制。本研究从蓖麻籽和药用坚果基因组中分别鉴定出26个和23个plcp基因，通过系统发育分析可将其划分为9个亚家族：RD21、CEP、XCP、XBCP3、Thi、SAG12、Rd19、ALP和CTB。虽然它们在拟南芥中大部分含有同源基因，但RD21、CEP、XBCP3和SAG12亚家族中的一些成员形成了新的组或亚组，这表明拟南芥中存在着特异性的基因缺失。最近在这两个物种中也发现了基因重复，但它们仅限于SAG12亚家族，都来自于局部复制。表达谱显示了不同家庭成员在不同组织上的不同模式。此外，还对plcp基因的进化特征进行了比较和讨论。我们的发现为研究大戟科及其他物种的plcp基因特征和家族进化提供了有用的参考。
Papain-like cysteine proteases (PLCPs) are a class of proteolytic enzymes involved in many plant processes. Compared with the extensive research inArabidopsis thaliana, little is known in castor bean (Ricinus communis) and physic nut (Jatropha curcas), two Euphorbiaceous plants without any recent whole-genome duplication. In this study, a total of 26 or 23 PLCP genes were identified from the genomes of castor bean and physic nut respectively, which can be divided into nine subfamilies based on the phylogenetic analysis: RD21, CEP, XCP, XBCP3, THI, SAG12, RD19, ALP and CTB. Although most of them harbor orthologs inArabidopsis, several members in subfamilies RD21, CEP, XBCP3 and SAG12 form new groups or subgroups as observed in other species, suggesting specific gene loss occurred inArabidopsis. Recent gene duplicates were also identified in these two species, but they are limited to the SAG12 subfamily and were all derived from local duplication. Expression profiling revealed diverse patterns of different family members over various tissues. Furthermore, the evolution characteristics of PLCP genes were also compared and discussed. Our findings provide a useful reference to characterize PLCP genes and investigate the family evolution in Euphorbiaceae and species beyond.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
5.1
作者：
Hierl, Georg;Hoewing, Timo;Isono, Erika;Lottspeich, Friedrich;Gietl, Christine
通讯作者：
Gietl, Christine
影响因子：
4.9
作者：
Chen, GH;Huang, LT;Chen, SCG
通讯作者：
Chen, SCG
影响因子：
14.9
作者：
Letunic I;Doerks T;Bork P
通讯作者：
Bork P
影响因子：
5.3
作者：
García-Lorenzo M;Sjödin A;Jansson S;Funk C
通讯作者：
Funk C