Protease gene families in Populus and Arabidopsis.
Protease gene families in Populus and Arabidopsis.
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DOI：
10.1186/1471-2229-6-30
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发表时间：
2006-12-20
影响因子：
5.3
通讯作者：
Funk C
中科院分区：
文献类型：
--
作者：
García-Lorenzo M;Sjödin A;Jansson S;Funk C
蛋白酶在植物中发挥着关键作用，维持严格的蛋白质质量控制，并在各种环境和发育刺激下降解特定的蛋白质组。不同物种中表达的蛋白酶之间的异同可能为其生理作用和进化提供有价值的见解。我们利用拟南芥MEROPS数据库中的蛋白酶编码基因，对拟南芥（Arabidopsis thaliana）和杨树（Populus trichocarpa）两个已测序的dicot基因组中的蛋白酶基因进行了比较分析，以确定杨树的同源序列。进行了多基因系统发育分析。大多数蛋白酶家族在杨树中比在拟南芥中更大，反映了最近的基因组复制。对DegP、Clp、FtsH、Lon、菱形和木瓜蛋白酶样蛋白酶家族的详细研究表明，基因家族扩展和基因丢失的模式是复杂的。我们最后表明，不同的杨树组织表达独特的蛋白酶基因套件，并且不同类别蛋白酶的mRNA水平沿发育梯度变化。近年来基因家族的扩张和收缩使得拟南芥和杨树的蛋白酶互补体不同，这与表达模式一起表明了蛋白酶的单个基因产物或组的作用。
Proteases play key roles in plants, maintaining strict protein quality control and degrading specific sets of proteins in response to diverse environmental and developmental stimuli. Similarities and differences between the proteases expressed in different species may give valuable insights into their physiological roles and evolution. We have performed a comparative analysis of protease genes in the two sequenced dicot genomes, Arabidopsis thaliana and Populus trichocarpa by using genes coding for proteases in the MEROPS database for Arabidopsis to identify homologous sequences in Populus. A multigene-based phylogenetic analysis was performed. Most protease families were found to be larger in Populus than in Arabidopsis, reflecting recent genome duplication. Detailed studies on e.g. the DegP, Clp, FtsH, Lon, rhomboid and papain-Like protease families showed the pattern of gene family expansion and gene loss was complex. We finally show that different Populus tissues express unique suites of protease genes and that the mRNA levels of different classes of proteases change along a developmental gradient. Recent gene family expansion and contractions have made the Arabidopsis and Populus complements of proteases different and this, together with expression patterns, gives indications about the roles of the individual gene products or groups of proteases.
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影响因子：
12.3
作者：
Andersson A;Keskitalo J;Sjödin A;Bhalerao R;Sterky F;Wissel K;Tandre K;Aspeborg H;Moyle R;Ohmiya Y;Bhalerao R;Brunner A;Gustafsson P;Karlsson J;Lundeberg J;Nilsson O;Sandberg G;Strauss S;Sundberg B;Uhlen M;Jansson S;Nilsson P
通讯作者：
Nilsson P
影响因子：
7.4
作者：
Chassin, Y;Kapri-Pardes, E;Adam, Z
通讯作者：
Adam, Z
影响因子：
4.8
作者：
Itzhaki, H;Naveh, L;Adam, Z
通讯作者：
Adam, Z
影响因子：
11.6
作者：
Heazlewood, JL;Tonti-Filippini, JS;Millar, AH
通讯作者：
Millar, AH
影响因子：
56.9
作者：
GAN, SS;AMASINO, RM
通讯作者：
AMASINO, RM