Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H(2) and CO(2) under different temperature conditions.
Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H(2) and CO(2) under different temperature conditions.
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嗜热甲烷杆菌的比较蛋白质组分析揭示了不同温度条件下 H-2 和 CO2 形成甲烷
DOI：
10.1002/mbo3.715
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发表时间：
2019-05
期刊：
影响因子：
3.4
通讯作者：
Ding X
中科院分区：
文献类型：
--
作者：
Liu C;Mao L;Zheng X;Yuan J;Hu B;Cai Y;Xie H;Peng X;Ding X
所有产甲烷菌的生长都被限制在特定的温度范围内。然而，甲烷热营养杆菌存在于各种自然和人工环境中，其温度有时甚至超过了嗜热菌的温度生长范围。因此，温度对甲烷产量和存活率的影响程度尚不清楚。为了研究古细菌进化以适应剧烈温度变化的产甲烷机制，利用相对和绝对定量等压标签(ITRAQ)研究了高温(71℃)和冷休克(4℃)条件下甲烷热营养杆菌对温度的响应。结果表明，高温和低温处理均能减少甲烷的生成，增加蛋白质的折叠和降解。此外，预计参与环境信息处理和细胞膜/壁/包膜生物发生的蛋白质可能在影响甲烷形成和增强嗜热单胞菌对温度胁迫的反应中发挥关键作用。对与这些温度相关蛋白对应的基因的基因组位置的分析预测，其中77个基因可能形成32个基因簇。在这里，我们评估了嗜热营养支原体对不同温度的反应，并提供了对甲烷形成和细胞假定的适应反应的新水平的理解。
The growth of all methanogens is limited to a specific temperature range. However, Methanothermobacter thermautotrophicus can be found in a variety of natural and artificial environments, the temperatures of which sometimes even exceed the temperature growth ranges of thermophiles. As a result, the extent to which methane production and survival are affected by temperature remains unclear. To investigate the mechanisms of methanogenesis that Archaea have evolved to cope with drastic temperature shifts, the responses of Methanothermobacter thermautotrophicus to temperature were investigated under a high temperature growth (71°C) and cold shock (4°C) using Isobaric tags for relative and absolute quantitation (iTRAQ). The results showed that methane formation is decreased and that protein folding and degradation are increased in both high‐ and low‐temperature treatments. In addition, proteins predicted to be involved in processing environmental information processing and in cell membrane/wall/envelope biogenesis may play key roles in affecting methane formation and enhancing the response of M. thermautotrophicus to temperature stress. Analysis of the genomic locations of the genes corresponding to these temperature‐dependent proteins predicted that 77 of the genes likely to form 32 gene clusters. Here, we assess the response of M. thermautotrophicus to different temperatures and provide a new level of understanding of methane formation and cellular putative adaptive responses.
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影响因子：
2.3
作者：
Ding, Xia;Yang, Wei-Jun;Lu, Zhen-Mei
通讯作者：
Lu, Zhen-Mei
影响因子：
5.1
作者：
Kato, Souichiro;Kosaka, Tomoyuki;Watanabe, Kazuya
通讯作者：
Watanabe, Kazuya
影响因子：
1.2
作者：
Chu, Pu;Yan, Gui Xia;Yang, Qing;Zhai, Li Na;Zhang, Cheng;Zhang, Feng Qi;Guan, Rong Zhan
通讯作者：
Guan, Rong Zhan
影响因子：
5.1
作者：
Campanaro, S.;Williams, T. J.;Cavicchioli, R.
通讯作者：
Cavicchioli, R.
影响因子：
7
作者：
Chen, Zhen;Wen, Bo;Liu, Siqi
通讯作者：
Liu, Siqi