Medium chain acylcarnitines dominate the metabolite pattern in humans under moderate intensity exercise and support lipid oxidation.
Medium chain acylcarnitines dominate the metabolite pattern in humans under moderate intensity exercise and support lipid oxidation.
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DOI：
10.1371/journal.pone.0011519
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发表时间：
2010-07-12
期刊：
影响因子：
3.7
通讯作者：
Niess AM
中科院分区：
文献类型：
--
作者：
Lehmann R;Zhao X;Weigert C;Simon P;Fehrenbach E;Fritsche J;Machann J;Schick F;Wang J;Hoene M;Schleicher ED;Häring HU;Xu G;Niess AM
运动是对骨骼肌能量代谢的极端生理挑战，具有显著的健康益处。我们的目的是识别和表征代谢物，这些代谢物是调节有益代谢适应运动的调节网络的组成部分。首先，我们调查了健康人类受试者的血浆，这些受试者完成了两项独立的中等有氧条件下的跑步研究。采用液相色谱- qtof质谱法，采用非靶向（NT-）代谢组学方法对运行前和运行后立即以及进入恢复阶段3和24 h的样品进行分析。在这些条件下，中链和长链酰基肉碱被发现是中等强度运动最具鉴别性的血浆生物标志物。在60分钟（93% VIAT）或120分钟（70% VIAT）后，立即观察到一个明显的，短暂的增加，主要是辛烷基，癸烷基和十二烷基-肉碱。通过检测13c -棕榈酸盐代谢，骨骼肌细胞培养实验证实了酰基肉碱作为部分β-氧化的中间体的释放。在人原发肌管和小鼠肌肉组织中的进一步研究表明，辛烷酰、癸烷酰和十二烷酰肉碱能够支持棕榈酸酯的氧化，证明比左旋肉碱更有效。通过功能代谢组学方法鉴定和表征中链酰基肉碱是中等强度运动中具有支持脂肪氧化能力的主要生物标志物。酰基肉碱的这种生理产生和外排可能在肌肉组织中发挥有益的生物学功能。
Exercise is an extreme physiological challenge for skeletal muscle energy metabolism and has notable health benefits. We aimed to identify and characterize metabolites, which are components of the regulatory network mediating the beneficial metabolic adaptation to exercise. First, we investigated plasma from healthy human subjects who completed two independent running studies under moderate, predominantly aerobic conditions. Samples obtained prior to and immediately after running and then 3 and 24 h into the recovery phase were analyzed by a non-targeted (NT-) metabolomics approach applying liquid chromatography-qTOF-mass spectrometry. Under these conditions medium and long chain acylcarnitines were found to be the most discriminant plasma biomarkers of moderately intense exercise. Immediately after a 60 min (at 93% VIAT) or a 120 min run (at 70% VIAT) a pronounced, transient increase dominated by octanoyl-, decanoyl-, and dodecanoyl-carnitine was observed. The release of acylcarnitines as intermediates of partial β-oxidation was verified in skeletal muscle cell culture experiments by probing 13C-palmitate metabolism. Further investigations in primary human myotubes and mouse muscle tissue revealed that octanoyl-, decanoyl-, and dodecanoyl-carnitine were able to support the oxidation of palmitate, proving more effective than L-carnitine. Medium chain acylcarnitines were identified and characterized by a functional metabolomics approach as the dominating biomarkers during a moderately intense exercise bout possessing the power to support fat oxidation. This physiological production and efflux of acylcarnitines might exert beneficial biological functions in muscle tissue.
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影响因子：
64.5
作者：
Cao H;Gerhold K;Mayers JR;Wiest MM;Watkins SM;Hotamisligil GS
通讯作者：
Hotamisligil GS
影响因子：
4.3
作者：
Enea, C.;Seguin, F.;Dugue, B.
通讯作者：
Dugue, B.
影响因子：
5
作者：
BENNETT, MJ
通讯作者：
BENNETT, MJ
影响因子：
13.2
作者：
Hoppel, C
通讯作者：
Hoppel, C
影响因子：
15.9
作者：
FRIOLET, R;HOPPELER, H;KRAHENBUHL, S
通讯作者：
KRAHENBUHL, S