Antioxidant components of naturally-occurring oils exhibit marked anti-inflammatory activity in epithelial cells of the human upper respiratory system.
Antioxidant components of naturally-occurring oils exhibit marked anti-inflammatory activity in epithelial cells of the human upper respiratory system.
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DOI：
10.1186/1465-9921-12-92
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发表时间：
2011-07-13
影响因子：
5.8
通讯作者：
Spannhake EW
中科院分区：
文献类型：
--
作者：
Gao M;Singh A;Macri K;Reynolds C;Singhal V;Biswal S;Spannhake EW
上呼吸道的功能是保护下呼吸道结构免受吸入空气中的化学和生物制剂的影响。导致急性和慢性炎症的细胞氧化应激在许多这些暴露中促成了所产生的病理学，并且是过敏性疾病、慢性鼻窦炎、污染物暴露以及细菌和病毒感染的典型。关于局部治疗鼻子可以加强其抗氧化和抗炎防御的有效方法，人们知之甚少。本研究旨在确定具有抗氧化活性的天然植物油是否可以提供可能发生上呼吸道保护的机制。在健康的人类受试者中进行上呼吸系统受控暴露于臭氧和鼻活检，以评估臭氧诱导的炎症反应的缓解，并评估由五种天然抗氧化油-芦荟、椰子、橙子、薄荷和维生素E的混合物诱导的鼻粘膜中的基因表达。BEAS-2B和NCI-H23上皮细胞系的细胞用于研究油诱导的抗炎活性的来源和潜在的细胞内作用机制。雾化预处理的混合油制剂显着衰减臭氧诱导的鼻腔炎症。尽管大多数油组分可通过还原来降低氧化应激，但已证明橙子油能够诱导与Nrf 2相关的几种抗氧化酶（包括HO-1、NQO 1、GCLm和GCLc）的持久基因表达，并减轻细胞培养系统中内毒素的促炎信号传导。证实了Nrf 2激活。在12名受试者中的9名中，雾化油制剂治疗增加了鼻粘膜HO-1表达的基线水平。这些数据表明，选择的油基抗氧化剂制剂可以有效地减少与氧化应激相关的鼻粘膜炎症。一些油激活细胞内抗氧化途径的潜力可能提供一种强大的机制，通过这种机制，可以在上呼吸道粘膜中提供有效和持久的细胞保护，防止空气环境暴露。
The upper respiratory tract functions to protect lower respiratory structures from chemical and biological agents in inspired air. Cellular oxidative stress leading to acute and chronic inflammation contributes to the resultant pathology in many of these exposures and is typical of allergic disease, chronic sinusitis, pollutant exposure, and bacterial and viral infections. Little is known about the effective means by which topical treatment of the nose can strengthen its antioxidant and anti-inflammatory defenses. The present study was undertaken to determine if naturally-occurring plant oils with reported antioxidant activity can provide mechanisms through which upper respiratory protection might occur. Controlled exposure of the upper respiratory system to ozone and nasal biopsy were carried out in healthy human subjects to assess mitigation of the ozone-induced inflammatory response and to assess gene expression in the nasal mucosa induced by a mixture of five naturally-occurring antioxidant oils - aloe, coconut, orange, peppermint and vitamin E. Cells of the BEAS-2B and NCI-H23 epithelial cell lines were used to investigate the source and potential intracellular mechanisms of action responsible for oil-induced anti-inflammatory activity. Aerosolized pretreatment with the mixed oil preparation significantly attenuated ozone-induced nasal inflammation. Although most oil components may reduce oxidant stress by undergoing reduction, orange oil was demonstrated to have the ability to induce long-lasting gene expression of several antioxidant enzymes linked to Nrf2, including HO-1, NQO1, GCLm and GCLc, and to mitigate the pro-inflammatory signaling of endotoxin in cell culture systems. Nrf2 activation was demonstrated. Treatment with the aerosolized oil preparation increased baseline levels of nasal mucosal HO-1 expression in 9 of 12 subjects. These data indicate that selected oil-based antioxidant preparations can effectively reduce inflammation associated with oxidant stress-related challenge to the nasal mucosa. The potential for some oils to activate intracellular antioxidant pathways may provide a powerful mechanism through which effective and persistent cytoprotection against airborne environmental exposures can be provided in the upper respiratory mucosa.
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影响因子：
10.4
作者：
Chen C;Arjomandi M;Balmes J;Tager I;Holland N
通讯作者：
Holland N
DOI：
10.1152/ajplung.1996.271.3.l383
发表时间：
1996-09-01
影响因子：
4.9
作者：
Choi, AMK;Knobil, K;Jacoby, DB
通讯作者：
Jacoby, DB
DOI：
10.1016/0165-5876(95)01133-v
发表时间：
1995-05-01
影响因子：
1.5
作者：
ARMENGOT, M;ESCRIBANO, A;BASTERRA, J
通讯作者：
BASTERRA, J
DOI：
10.1164/rccm.200706-863oc
发表时间：
2008-02-15
影响因子：
24.7
作者：
Islam, Talat;McConnell, Rob;Gilliland, Frank D.
通讯作者：
Gilliland, Frank D.
DOI：
10.1073/pnas.93.19.10393
发表时间：
1996-09-17
影响因子：
11.1
作者：
Lee, PJ;Alam, J;Choi, AMK
通讯作者：
Choi, AMK