Molecular Characterization of bla (IMP) (-) (4) -Carrying Enterobacterales in Henan Province of China.
Molecular Characterization of bla (IMP) (-) (4) -Carrying Enterobacterales in Henan Province of China.
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DOI：
10.3389/fmicb.2021.626160
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发表时间：
2021
影响因子：
5.2
通讯作者：
Qin S
中科院分区：
文献类型：
--
作者：
Liu W;Dong H;Yan T;Liu X;Cheng J;Liu C;Zhang S;Feng X;Liu L;Wang Z;Qin S
耐碳青霉烯类肠球菌对临床管理和公共卫生构成严重威胁。对12株IMP-4金属β-内酰胺酶产生菌进行了分子生物学特性研究，包括5株阴沟肠杆菌、3株大肠埃希菌、2株肺炎克雷伯菌和2株弗氏柠檬酸杆菌。这些菌株于2013年至2015年从郑州的一家三级教学医院收集。最低抑菌浓度（MIC）结果显示，blaIMP-4阳性菌株均为多药耐药（MDR），但对粘菌素敏感。所有的E。coli为ST 167，2株C.弗氏菌株属于ST 396型，E. cloxacin和K.肺炎。S1-PFGE、Southern印迹和基于PCR的复制子分型分析显示，携带blaIMP-4的质粒范围为0.52至0.360 kb，属于PHI、FIB、HI 2/HI 2A和N型。N质粒占优势（8/12，66.7%）。质粒稳定性试验表明，携带blaIMP-4的N型质粒比其他类型的质粒更稳定。接合试验表明，三个blaIMP-4携带的N质粒是可转移的。对代表性的N型（pIMP-ECL 14 -57）的全序列分析表明，它与携带C.弗氏菌株PCR图谱分析表明，转座子样结构[IS 6100-mobC-intron（K1.pn.I3）-blaIMP-4-IntI 1-IS 26]在所有N质粒中高度保守。IS 26涉及重组事件，其导致该转座子样模块在FIB和FIB质粒中的可变结构。blaIMP-4基因被HI 2/HI 2A质粒pIMP-ECL-13-46上的sul 1型整合子In 1589捕获。
Carbapenem-resistant Enterobacterales (CRE) pose a serious threat to clinical management and public health. We investigated the molecular characteristics of 12 IMP-4 metallo-β-lactamase-producing strains, namely, 5 Enterobacter cloacae, 3 Escherichia coli, 2 Klebsiella pneumoniae, and 2 Citrobacter freundii. These strains were collected from a tertiary teaching hospital in Zhengzhou from 2013 to 2015. The minimum inhibitory concentration (MIC) results showed that each blaIMP–4-positive isolate was multidrug-resistant (MDR) but susceptible to colistin. All of the E. coli belonged to ST167, two C. freundii isolates belonged to ST396, and diverse ST types were identified in E. cloacae and K. pneumoniae. S1-PFGE, Southern blotting, and PCR-based replicon typing assays showed that the blaIMP–4-carrying plasmids ranged from ∼52 to ∼360 kb and belonged to FII, FIB, HI2/HI2A, and N types. N plasmids were the predominant type (8/12, 66.7%). Plasmid stability testing indicated that the blaIMP–4-carrying N-type plasmid is more stable than the other types of plasmids. Conjugative assays revealed that three of the blaIMP–4-carrying N plasmids were transferrable. Complete sequence analysis of a representative N type (pIMP-ECL14–57) revealed that it was nearly identical to pIMP-FJ1503 (KU051710) (99% nucleotide identity and query coverage), an N-type blaIMP–4-carrying epidemic plasmid in a C. freundii strain. PCR mapping indicated that a transposon-like structure [IS6100-mobC-intron (K1.pn.I3)-blaIMP–4-IntI1-IS26] was highly conserved in all of the N plasmids. IS26 involved recombination events that resulted in variable structures of this transposon-like module in FII and FIB plasmids. The blaIMP–4 gene was captured by a sul1-type integron In1589 on HI2/HI2A plasmid pIMP-ECL-13–46.
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影响因子：
5.2
作者：
Jin C;Zhang J;Wang Q;Chen H;Wang X;Zhang Y;Wang H
通讯作者：
Wang H
DOI：
10.1186/s12941-017-0218-9
发表时间：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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