Establishment of a detection assay for DNA endonuclease activity and its application in the screening and prognosis of malignant lymphoma.
Establishment of a detection assay for DNA endonuclease activity and its application in the screening and prognosis of malignant lymphoma.
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DNA核酸内切酶活性检测方法的建立及其在恶性淋巴瘤筛查和预后中的应用
DOI：
10.1186/s12858-018-0096-6
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发表时间：
2018-07-31
期刊：
影响因子：
--
通讯作者：
Guo L
中科院分区：
文献类型：
--
作者：
Lu R;Wang Y;Xu X;Xie S;Wang Y;Zhong A;Zheng H;Yu Y;Gao X;Guo L
背景核酸内切酶在维持基因组稳定性和调节细胞生长中起着重要作用。本研究的目的是评估检测的核酸内切酶活性作为一个指标，在早期诊断和预后的lymphoma.ResultsThe方法的核酸内切酶活性测定成功地建立和应用于比较癌症患者和对照cohols.ResultsThe。癌组织中核酸内切酶活性显著高于癌旁组织（P< 0.001）。我们接下来研究了入组患者和对照的外周血中的核酸内切酶活性，患者中的核酸内切酶活性也显著高于对照（P= 0.015）。转移组与非转移组相比，核酸内切酶活性明显升高（P= 0.038），但在年龄（≤ 56岁，> 56岁）和性别之间差异无统计学意义（分别为P= 0.736 > 0.05和P = 0.635 > 0.05）。对照组与非转移性恶性肿瘤患者间差异无统计学意义（P= 0.800 > 0.05），但与非转移性恶性肿瘤伴淋巴瘤患者间差异有统计学意义（P= 0.033）。R比值≥ 1.4者的无进展生存率明显低于R比值<1.4者。结论建立了一种检测核酸内切酶活性的方法，可用于恶性肿瘤尤其是淋巴瘤的预后判断。
BackgroundEndonucleases play critical roles in maintaining genomic stability and regulating cell growth. The purpose of this study was to evaluate detection of endonuclease activity as an indicator in the early diagnosis and prognosis of lymphoma.ResultsThe method of endonuclease activity determination was successfully established and applied to compare cancer patient and control cohorts. Endonuclease activities of cancer tissues were significantly higher than those of adjacent control tissues (P< 0.001). We next investigated endonuclease activity in peripheral blood of enrolled patients and the controls, which were also significantly higher in patients than in controls (P= 0.015). Additionally, endonuclease activities were elevated in the metastasis subgroup compared with the non-metastasis subgroup(P= 0.038), whereas no significant difference was found between age(≤ 56y, > 56y) and gender (P= 0.736 > 0.05 andP= 0.635 > 0.05, respectively). Although there was no significant difference between control group with the non-metastatic cancer patients (P= 0.800 > 0.05), endonuclease activities were lower in the control group compared with the non-metastatic cancer patients with lymphoma (P= 0.033). The progression-free survival probability of patients with elevated R ratios(R ratio ≥ 1.4) was significantly lower than that of patients with lower R ratios (R ratio < 1.4).ConclusionsAn assay was established to detect the endonuclease activity,which might be useful for the prognosis of cancers, especially lymphoma.
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影响因子：
5.1
作者：
Chen, Wan-qing;Zeng, Hong-mei;He, Jie
通讯作者：
He, Jie
影响因子：
3.7
作者：
Kim SG;Kim DM;Woo JT;Jang HC;Chung CH;Ko KS;Park JH;Park YS;Kim SJ;Choi DS
通讯作者：
Choi DS
影响因子：
5.3
作者：
Wang, Dong;Xiang, De-Bing;Zhang, Yun-Song
通讯作者：
Zhang, Yun-Song
影响因子：
1.2
作者：
Ren, Hongtao;Ma, Hongbing;Dai, Zhi-Jun
通讯作者：
Dai, Zhi-Jun
影响因子：
8
作者：
Saharia, A.;Stewart, S. A.
通讯作者：
Stewart, S. A.