Preparation of immobilized arylsulfatase on magnetic Fe(3)O(4) nanoparticles and its application for agar quality improvement.
Preparation of immobilized arylsulfatase on magnetic Fe(3)O(4) nanoparticles and its application for agar quality improvement.
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DOI：
10.1002/fsn3.2446
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发表时间：
2021-09
影响因子：
3.9
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Zhang C;Jiang Z;Li H;Ni H;Zheng M;Li Q;Zhu Y
琼脂中硫酸盐基团的存在通过影响胶凝过程中的交联而损害琼脂质量。一些芳基硫酸酯酶可以催化琼脂中硫酸根键的水解，从而改善琼脂的品质。固定化芳基硫酸酯酶证明了其工业应用的有益优点。在这里，一个先前表征的突变芳基硫酸酯酶K253 H/H260 L固定在合成的磁性Fe 3 O 4纳米粒子功能化后，由单宁酸（MNP @TA）。用扫描电镜和傅里叶变换红外光谱研究了固定化芳基硫酸酯酶（MNP @TA@ARS）的表面性质和分子结构。酶促特性研究表明，MNP @TA@ARS的最适温度和pH发生了变化，其表观Km和Vmax与游离MNP相比发生了变化。固定化芳基硫酸酯酶表现出改善的热和pH稳定性和增强的储存稳定性与适度的可重复使用性。此外，MNP @TA@ARS显示出对各种抑制剂和洗涤剂的耐受性增强。利用固定化芳基硫酸酯酶处理琼胶，可提高琼胶的质量。将海洋细菌来源的芳基硫酸酯酶固定在磁性纳米珠上。固定化芳基硫酸酯酶具有增强的热稳定性和pH稳定性。琼脂经固定化酶处理后，其质量与琼脂糖相当。
The presence of sulfate groups in agar compromises the agar quality by affecting the crosslinking during gelling process. Some arylsulfatases can catalyze the hydrolysis of sulfate bonds in agar to improve the agar quality. Immobilized arylsulfatases prove beneficial advantages for their industrial applications. Here, a previously characterized mutant arylsulfatase K253H/H260L was immobilized on the synthesized magnetic Fe3O4 nanoparticles after functionalization by tannic acid (MNPs@TA). The surface properties and molecular structures of the immobilized arylsulfatase (MNPs@TA@ARS) were examined by scanning electron microscopy and Fourier transform infrared spectroscopy. Enzymatic characterization showed that MNPs@TA@ARS exhibited shifted optimal temperature and pH with deviated apparent Km and Vmax compared to its free counterpart. The immobilized arylsulfatase demonstrated improved thermal and pH stability and enhanced storage stability with modest reusability. In addition, MNPs@TA@ARS displayed enhanced tolerance to various inhibitors and detergents. The utilization of the immobilized arylsulfatase for agar desulfation brought the treated agar with improved quality. An arylsulfatase of marine bacterial origin was immobilized on the magnetic nanobeads. The immobilized arylsulfatase had enhanced thermal and pH stability. After treatment of agar by the immobilized enzyme, agar had equivalent quality to agarose.
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